鸢尾科红葱红色素的提取及抗氧化活性评价

Extraction and Antioxidant Activity of Red Pigments from Eleutherine americana

在单因素试验的基础上,采用二次回归正交旋转组合试验对超声辅助提取鸢尾科红葱红色素的工艺条件进行优化,并对红葱红色素粗提物的体外抗氧化活性进行评价。结果表明,红葱红色素最优提取工艺条件为:乙醇体积分数31%,料液比1∶54(g/mL),超声时间40 min;在此条件下,红葱红色素粗提物得率为17.97%。红葱红色素粗提物具有一定的还原能力、DPPH自由基清除能力和羟自由基清除能力,其清除效果与市售红曲红色素接近,清除能力与浓度之间呈现一定的量效关系。

Based on single factor experiment, quadratic regression orthogonal rotation combination design method was used to optimize the ultrasound-assisted extraction conditions of red pigments from Eleutherine americana. The antioxidant activity of the crude extraction was evaluated. The results showed that the optimal extraction conditions were as follows： enthnol concentration 31%, sample/solvent ratio 1 ： 54 （g/mL） and ultrasonic time 40 min. Under the optimal conditions, the crude extract yield of red pigments from Eleutherine americana was 17.97%. The crude extraction exhitited moderate reducing capacity and radical scavenging activity on against 1, 1-diphenyl-2-picrylhydrazyl （DPPH） free radical and hydroxyl radical. Its radical scavenging effect was similar to that of monascus red pigments commercially available and the scavenging activity was proportional to the concentration of the extract.