TLR4基因多态性与变应性鼻炎的相关性研究

Correlation between TLR4 gene polymorphisms and allergic rhinitis

目的:探讨TLR4基因多态性与变应性鼻炎(AR)易感性的关系。方法:以212例AR患儿和169例健康对照儿童为研究对象,选取TLR4基因的rs10759930、rs2737190和rs2770150位点,以聚合酶链反应测序法完成基因型检测,以SPSS 17.0软件进行统计学分析,调查TLR4基因多态性位点分布特征,对AR和健康对照组儿童TLR4基因多态性位点的等位基因频率和基因型频率进行比较。结果:位点rs10759930的CT杂合(P=2.70×10^(-6),OR=2.66,95%CI1.76～4.00)及TT纯合突变(P=9.72×10^(-9),OR=8.78,95%CI 3.83～20.13)与AR具有相关性。结论:rs10759930的CT杂合及TT纯合突变均与AR的发病相关。

Objective：To investigate the correlation between polymorphisms in the TLR4 locus and the susceptibility of allergic rhinitis. Method.. Three polymorphisms in TLR4 locus（rs10759930, rs2737190 and rs2770150） were genotyped in 212 subjects with allergic rhinitis andin 169 healthy controls. PCR sequencing methods was performed for genotyping. SPSS program version 17.0 was used for statistic analysis. The characteristic of genetic polymorphism was analyzed. The differences of genotype and allele frequencies between the two groups of children were compared. Result： heterozygous mutations CT（P=2.70 ）〈 10-6, OR= 2.66,95% CI： 1.76- 4.00）, and homozygous mutation TT（P= 9.72 5〈 10.9 , OR= 8.78,95 % CI= 3.83 - 20. 13） of rs10759930 appeared significant, are related to AR. Conclusion： Heterozygous and homozygous allele in rs10759930 is associated with allergic rhinitis.