莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制研究

Study on the Preventive and Therapeutic Effect and Its Mechanism of Medicine Pair Decoction Liquid of Curcuma phaeocaulis-Sparganium stoloniferum on Uterine Myoma in Rats

目的:研究莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制。方法:将大鼠随机分为正常组、模型组、阳性组(米非司酮,2.25 mg/kg)和药对合煎液组(莪术-三棱药对合煎液,6.0 g/kg),每组10只。除正常组外,其余各组大鼠分别于每周一、三、五im苯甲酸雌二醇注射液(0.5 mg/kg),持续12周;从第13周开始,造模组大鼠增加im黄体酮注射液(5 mg/kg),并每天ig给药1次,至实验第16周。给药结束后,测定大鼠子宫系数,苏木精-伊红染色观察大鼠子宫病理变化并测定平滑肌厚度,免疫组化染色法测定大鼠子宫组织中转化生长因子β_3(TGF-β_3)和基质金属蛋白酶11(MMP-11)蛋白表达。结果:与正常组比较,模型组大鼠子宫系数升高、子宫发生明显病理变化、子宫平滑肌厚度增加、子宫组织中TGF-β_3和MMP-11蛋白表达增强(P<0.05或P<0.01);与模型组比较,阳性组和药对合煎液组大鼠上述变化均显著改善(P<0.05或P<0.01)。结论:莪术-三棱药对合煎液对大鼠子宫肌瘤有一定的防治作用,其机制可能与下调子宫组织中TGF-β_3、MMP-11蛋白表达有关。

OBJECTIVE：To study the preventive and therapeutic effect and its mechanism of medicine pair decoction liquid of Curcuma phaeocaulis-Sparganium stoloniferum on rats with uterine myoma. METHODS：Rats were randomly divided into normal group,model group,positive group（mifepristone,2.25 mg/kg）and medicine pair decoction liquid group（C. phaeocaulis-S. stoloniferum decoction liquid,6.0 g/kg）,10 in each group. Except for the normal group,rats in other groups were injected Estradiol benzoate injection（0.5 mg/kg） intramuscularly every Monday,Wednesday and Friday,for 12 weeks. From the 13 th week,rats in modeling group were added Progesterone injection（5 mg/kg）intramuscularly as well as relevant medicines intragastrically,once a day,until the 16 th week. After administration,uterine coefficient of rats was detected. HE staining was used to observe the pathological changes of uterus and determine the thickness of smooth muscle;immunohistochemical staining was adopted to detect the transforming growth factor β3（TGF-β3）,matrix metalloproteinase 11（MMP-11）protein expressions in uterus tissue of rats. RESULTS：Compared with normal group,uterine coefficient was increased in model group,pathological changes were obvious in uterus,thickness of smooth muscle was increased,TGF-β3and MMP-11 protein expressions were enhanced（P〈0.05 or P〈0.01）.Compared with model group,above-mentioned changes were improved significantly in positive group and medicine pair decoction liquid groups（P〈0.05 or P〈0.01）. CONCLUSIONS：Medicine pair decoction liquid of C. phaeocaulis-S. stoloniferum shows certain preventive and therapeutic effect on rats with uterine myoma. The mechanism may be associated with downregulating the TGF-β3,MMP-11 protein expressions in uterus tissue.