螺旋藻激酶对动脉粥样硬化模型大鼠血管内皮功能的影响

Effects of Spirulina Kinase on Vascular Endothelial Function of Model Rats with Atherosclerosis

目的:研究螺旋藻激酶(SPK)对动脉粥样硬化模型大鼠血管内皮功能的影响。方法:将60只大鼠随机分为正常对照组(蒸馏水)、模型组(蒸馏水)、阳性对照组(辛伐他汀,0.005 g/kg)和SPK低、中、高剂量组(80、160、320 U/kg)。除正常对照组外,其余各组大鼠均复制动脉粥样硬化模型;同时,各组大鼠ig相应药物,每天1次,连续给药12周。测定大鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量;苏木精-伊红染色观察大鼠胸主动脉血管内膜的形态改变。结果:与正常对照组比较,模型组大鼠血清中TC、TG、LDL-C、IL-6和TNF-α含量增加(P<0.01),HDL-C含量降低(P<0.01);血管内皮细胞脱落,内膜增生、向管腔内突起,平滑肌细胞增殖且排列紊乱,中膜弹力纤维崩解断裂。与模型组比较,各给药组大鼠血清中TC、TG、LDL-C、IL-6和TNF-α含量下降(P<0.05或P<0.01),阳性对照组和SPK中、高剂量组大鼠血清中HDL-C含量增加(P<0.05);给药组大鼠血管内皮细胞形态较模型组明显改善,其中SPK中、高剂量组大鼠血管内皮细胞各层结构完整、内膜基本光滑,SPK中剂量组大鼠血管中膜平滑肌细胞排列稍紊乱,与正常对照组比较无明显变化。结论:SPK有明显的降脂和抗炎作用,可保护血管内皮功能;其作用机制可能与降低血清中TC、TG、LDL-C、IL-6、TNF-α含量和升高血清中HDL-C含量有关。

OBJECTIVE：To study the effects of spirulina kinase（SPK）on the vascular endothelial function of model rats with atherosclerosis. METHODS：60 rats were randomly divided into normal control group（distilled water）,model group（distilled water）,positive control group（simvastatin,0.005 g/kg）and SPK low-dose,medium-dose,high-dose groups（80,160,320 U/kg）.Except for normal control group,rats in other groups were induced for model of atherosclerosis. All groups were intragastrically administrated relevant medicines at the same time,once a day for consecutive 12 weeks. Total cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C）,interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α）contents in serum of rats were measured. And the changes of thoracic aortic endothelium morphology were observed by HE staining. RESULTS：Compared with normal control group,TC,TG,LDL-C,IL-6,TNF-α contents in serum of rats in model group were increased（P〈0.01）,HDL-C content in serum was decreased（P〈0.01）;vascular endothelial cells fell off,intimal proliferation projected into the lumen,smooth muscle cell proliferated and disordered,medium film elastic fiber disintegrated and fractured. Compared with model group,TC,TG,LDL-C,IL-6,TNF-α contents in serum of rats in administration groups were decreased（P〈0.05 or P〈0.01）,HDL-C content in positive control group and SPK medium-dose,high-dose groups was increased（P〈0.05）. Vascular endothelial cell morphology was improved significantly in administration groups,in which,vascular endothelial cells were structurally intact in SPK medium-dose,high-dose groups,inner membrane was basically smooth;medium smooth muscle cells arranged slightly disordered in SPK medium-dose group. Compared with normal control group,there were no obvious changes. CONCLUSIONS：SPK shows obvious lipid-lowering and anti-inflammatory effects,it can protect vascular endothelial function. The mechanism may be related to reducing TC,TG,LDL-C,HDL-C,IL-6,TNF-α contents and increasing HDL-C content in serum.