正交试验优化独子藤果实中雷公藤红素的醇提工艺

Optimization of Methanol Extraction Technology for Tripterine in the Fruit of Celastrus monospermus by Orthogonal Design

目的:优选独子藤果实中雷公藤红素的醇提工艺。方法:采用高效液相色谱法测定雷公藤红素的含量。以提取时间、提取次数、料液比为考察因素,以雷公藤红素的提取率为考察指标设计正交试验,并进行验证试验。结果:最优醇提工艺为45%甲醇提取3次、每次2 h、料液比1∶8;验证试验中,雷公藤红素的平均提取率为0.917 mg/g(RSD=2.85%,n=3)。结论:本研究优化的醇提工艺稳定可行、提取率较高,适用于独子藤果实中雷公藤红素的提取。

OBJECTIVE：To optimize the methanol extraction technology of tripterine in the fruit of Celastrus monospermus.METHODS：HPLC was adopted to determine the content of tripterine. Using extraction time,extraction times,solid-liquid ratio as investigation factors,extraction rate of tripterine as investigation index,orthogonal test was designed,and verification test was conducted. RESULTS：The optimal methanol extraction technology was as follow as 45% methanol extracting 2 h each time with solidliquid ratio of 1∶8 for 3 times. In the verification test,the average extraction rate of tripterine was 0.917 mg/g（RSD=2.85%,n=3）. CONCLUSIONS：The optimized methanol extraction technology is stable and feasible with high extraction rate,and is suitable for the extraction of tripterine in the fruit of C. monospermus.