Paenibacillus bovis sp.nov.BD3526作为益生菌的潜力

Assessment of Paenibacillus bovis sp. nov. BD3526 as a potential probiotics in vitro

对Paenibacillus bovis sp.nov.BD3526作为益生菌的潜力进行了体外活性初步研究。采用模拟胃液、肠液、不同浓度的胆盐环境,测定BD3526菌株在酸性及高胆盐环境的存活率,并测试BD3526对金黄色葡萄球菌、藤黄、枯草芽孢杆菌的抑制作用。结果表明,BD3526可以耐受pH值为2.0、3.0、4.0的人工胃液、pH8.0的模拟肠液以及0.1%、0.2%、0.3%的胆盐环境;对金黄色葡萄球菌、藤黄、枯草芽孢杆菌有显著的抑制能力。说明BD3526在人工消化液环境中具有良好的存活能力,不具有溶血作用;可引起敏感菌细胞渗漏,达到杀灭部分细菌,包括金色葡萄球菌的作用,因此,该菌具备作为益生菌应用的潜力。

To investigate the potential of Paenibacillus bovis sp. nov. BD3526 as a probiotics in vitro, the growth of the strain BD3526 in different media, i.e. TYC and BHI broths was investigated. The survivals of BD3526 cells under different levels of bile salts （0.1% to 0.3% （w/v））, in simulated gastric juice with varied pH values （pH 2 to 4） and in simulated intestinal juice were determined. Furthermore, the mechanism involved in the antagonistic ac- tivity of the strain BD3526 against Staphylococcus aureus through lysis of the indicator cells was also assayed. BD3526 displayed strong antagonistic activity to some bacteria including opportune pathogen, e.g. Staphylococcus aureus and good survival ability in simulated gastric juice as well as intestinal juice. No hemolytic activity was observed for the strain BD3526 on blood agar. Paenibacillus bovis sp. nov. BD3526 was demonstrated to be a potential probiotics.