海洋脂肪酶YS2071的固定化及酶学性质研究

The immobilization and enzymatic properties of lipase YS2071

为提高脂肪酶的利用效率,更好应用于工业化生产,研究了脂肪酶YS2071的固定化技术,筛选最优载体,通过单因素实验法对脂肪酶固定化的条件进行优化,并对其性质进行了研究。采用MI-BSI伯胺功能基吸附树脂为载体,京尼平为交联剂,吸附-交联的方法,分析了加酶量、吸附时间、吸附温度、初始pH、交联剂的浓度、交联时间等因素对脂肪酶固定化效果的影响,并通过PB实验和响应面实验,确定了最佳固定化条件:加酶量为8 mg,吸附时间8 h,吸附温度为20℃,初始pH 8.6,交联剂质量浓度为0.48 g/L,交联时间为1 h时,固定化效率最高,酶活回收率达到60%以上。

Free lipase is difficult to achieve continuous operation. In order to improve the efficiency of lipase and apply it to the industrialized production, the technology of immobilized lipase YS2071 was studied by screening the optimal carrier and using single factor experiment method to optimize lipase immobilized conditions. Using MI-BSI res- in as carrier and genipin as cross-linking agent, adsorption-crosslinking method was employed to maintain relative high enzyme activity. Immobilized conditions were studied by using PB experiment and the response surface design. The optimal conditions of immobilized lipase YS2071 were as followes： the enzyme dosage was 8 mg, the adsorption time was 8 h, the adsorption temperature was 20 ℃ , the initial pH was 8.6, the concentration of genipin was 0.48 g/L, the crosslinking time was 1 h. Under these conditions, the recovery rate of immobilized enzyme was up to 60%.