摘要
目的:建立樱桃多糖含量的测定方法,并进行樱桃多糖、水不溶性固形物、水分的测定,初步对樱桃的质量进行评价。方法:以硫酸—蒽酮显色,采用紫外—分光光度法测定,检测波长为627 nm;水不溶性固形物采用国际标准ISO 751—1981《水果、蔬菜制品—水不溶性固形物测定法》测定;水分测定按照《中国药典》2010版附录Ⅸ水分测定法(第一法)测定。结果:樱桃多糖的线性范围为0.0392~0.196 mg(r=0.9994);11个产地樱桃多糖的平均含量为0.99%,其中兴义含量最高,六枝含量最低;水不溶性固形物平均含量为2.87%,其中乌当下坝含量最高,六枝含量最低;水分的平均含量为86.05%,其中赫章水份含量最高,镇宁水份含量最低。结论:建立的多糖的测定方法操作简单,重现性好,可用于樱桃多糖的含量测定;不同产地的樱桃中多糖、水不溶性固形物、水分的含量有一定的差异性,为贵州樱桃质量标准的制定提供参考。
Objective：To determine the contents of polysaccharides,water-insoluble solids,and moisture in different areas of cherry in Gui Zhou,the quantitative research of cherry was preliminarily studied. Methods：The content of Polysaccharide was tested with the UV spectrophotometry and color appearing in thesulfuric acid-anthrone reagent. the testing wavelength was 627 nm. Water-insoluble solids using international standards—ISO751-1981 ＂ Fruit and vegetable products – measurement of water-insoluble solids＂. And moisture accordance with the ＂ Chinese Pharmacopoeia ＂ 2010 version of Appendix Ⅸ determination of moisture content（ first method）. Results：The linear range of cherry polysaccharide was 0. 0392 ~ 0. 196 mg（ r = 0. 9994）; The average polysaccharide in cherries which from 11 different areas was 0. 99%,in which the highest Moisture content is Xing Yi while the minimum is Liu Zhi; water-insoluble solids content of 2. 87% on average,among the highest content of black current dam,Liuzhi with minimum content;the average moisture content of 86. 05%,among the He Zhang which has the highest moisture content,Zhen Ning with the minimum. Conclusion： The method is simple,accurate and reproducible,and can be used to determine the polysaccharides in cherry. The contents of polysaccharides,water-insoluble solids,and moisture were different in different areas,which proving areference quality standards for Gui Zhou cherries.
出处
《微量元素与健康研究》
CAS
2017年第4期48-51,共4页
Studies of Trace Elements and Health
基金
黔科合重大专项字[2015]6009-3
国家苗药工程技术研究中心(2014FU125Q09)
