毛细管电泳噪声特性分析

Analysis of Capillary Electrophoresis Noise Characteristics

为优化毛细管电泳荧光信号检测系统,提高检测灵敏度,以100~1 000碱基对的脱氧核糖核酸作为分离对象,羟乙基纤维素为筛分介质,研究了直流电场下毛细管电泳荧光信号检测系统中的噪声特性.对不同分离电场强度、羟乙基纤维素溶液浓度和分子量、毛细管有效长度以及毛细管内径形状等情况下的噪声特性进行分析.分析得到该检测系统中信噪比最佳的优化参量,即分离电场强度为500~600V/cm、羟乙基纤维素浓度为0.6%~0.7%、羟乙基纤维素分子量为250、圆形内径为50μm以及毛细管有效长度为8cm.

In order to optimize the fluorescence detection capillary electrophoresis system and improve the detection sensitivity,the signal noise characters were studied when separating 100~1 000 base pair deoxyribonucleic acid ladder under direct current electric field in hydroxyethyl cellulose solutions.The dependence of signal noise on separation electric field intensity,polymer concentration,hydroxyethyl cellulose molecular weight,the effective length and the capillary shape were investigated.Experiment result shows that optimum parameters can be obtained under best signal noise ratio,which are the separation electric field strength of 500~600V/cm,hydroxyethyl cellulose concentration of 0.6%~0.7%、hydroxyethyl cellulose molecular weight of 250,round inner diameter of 50μm and effective capillary length of 8cm.