藏药佐太中汞在小鼠体内的蓄积

Mercury accumulation of Tibetan medicine Zuotai in mice

目的探索给予KM小鼠不同剂量和不同时间的佐太后,汞在体内的蓄积情况。方法 KM小鼠随机分为空白组,佐太低、中、高剂量组(42 d,6.07、60.70、606.97 mg/kg;14 d,606.97 mg/kg),给药后,测定小鼠脑(嗅球、皮层、海马、下丘脑、脑干、小脑)、心脏、肺脏、肾脏、肝脏、脾脏、血清、肌肉中汞含有量。结果与空白组相比,给予42 d低剂量佐太后,能够显著增加小鼠海马、小脑、肺脏、肾脏、肝脏、血清中汞含有量;给予42 d中剂量佐太后,能够显著增加小鼠嗅球、皮层、海马、脑干、小脑、心脏、肺脏、肾脏、肝脏、脾脏、血清中汞含有量;给予42、14 d高剂量佐太后,能够显著增加小鼠嗅球、皮层、海马、下丘脑、脑干、小脑、心脏、肺脏、肾脏、肝脏、脾脏、肌肉、血清中汞含有量。结论小鼠灌胃佐太后,汞在不同组织中产生蓄积,且表现出剂量和时间依赖性,提示佐太及其复方制剂不宜过量、长期使用。

AIM To explore the mercury accumulation in KM mice after being given Zuotai at different doses and time. METHODS KM mice were randomly divided into blank group, Zuotai low-, middle- and high-dose （6.07, 60.70 and 606.97 mg/kg, 42 d; 606.97 mg/kg, 14 d） groups. The mercury contents in brain （olfactory bulb, cortex, hippocampus, hypothalamus, brain stem, cerebellum）, heart, lung, kidney, liver, spleen, serum, muscle of mice were measured after administration. RESULTS Compared with the blank group, Zuotai at low- dose significantly increased the mercury contents in hippocampus, cerebellum, lung, kidney, liver and serum of mice after 42-day treatment; Zuotai at middle-dose markedly increased the mercury contents in olfactory bulb, cor- tex, hippoeampus, brain stem, cerebellum, heart, lung, kidney, liver, spleen and serum of mice after 42-day treatment; the mice treated with high-dose of Zuotai for 42, 14 days significantly increased the mercury contents in olfactory bulb, cortex, hippocampus, hypothalamus, brain stem, cerebellum, heart, lung, kidney, liver, spleen, muscle and serum. CONCLUSION Mercury can be accumulated in different tissues of mice after intragastric ad- ministration of Zuotai in a dose- and time-dependent manner, which suggests that Zuotai and its compound prepara- tions should not be used in high-dose and long-term.