人脐血间充质干细胞移植对脑梗死兔神经功能障碍和头部核磁共振波谱的影响

Effect of human umbilical cord blood mesenchymal stem cells transplantation on functional recovery and the ~1H-MRS parameters in the rabbits with cerebral ischemia-reperfusion

目的观察人脐血间充质干细胞移植对缺血再灌注兔神经功能障碍和头部核磁共振波谱参数的影响。方法采集足月新生儿脐带血60~80 ml,分离h UCB-MSCs,并体外培养。将造模成功的大脑中动脉栓塞模型30只兔随机分为脑梗死组、干细胞组、生理盐水组,每组10只,后两组移植人脐血间充质干细胞和生理盐水。移植48 h、2 w时,采用Purdy评分进行神经损害严重程度评估,行核磁共振及氢质子核磁共振波谱检查,进行梗死体积、及梗死中心区N-乙酰天门冬氨酸、胆碱和肌酸等代谢物含量的测定。结果与脑梗死组及生理盐水组相比,干细胞组NSS评分较低,差异具有统计学意义(P<0.05);生理盐水组与脑梗死组NSS评分无明显差异(P>0.05)。与脑梗死组和生理盐水组相比,干细胞组梗死体积明显减小(P<0.05)。与假手术组相比,干细胞组、脑梗死组与生理盐水组NAA/Cr、Cho/Cr比值均明显下降,Lac/Cr比值均显著上升(P<0.01);但干细胞组NAA/Cr、Cho/Cr比值显著高于脑梗死组及生理盐水组(P<0.05),Lac/Cr比值显著低于后两组(P<0.05);而生理盐水组NAA/Cr、Cho/Cr、Lac/Cr比值与脑梗死组之间无明显差异(P>0.05)。结论脐血间充质干细胞移植能提高脑梗死兔脑NAA/Cr、Cho/Cr比值,降低Lac/Cr比值,缩小梗死范围,明显改善兔神经功能。

Objective To observe the effect of human umbilical cord blood mesenchymal stem cells（ h UCB-MSCs）transplantation on functional recovery and the -1H-MRS parameters in the rabbits with cerebral ischemia-reperfusion.Methods The h UCB-MSCs abstracted from 60 - 80 ml of cord blood of full term babies were cultured in vitro. The cerebral artery occlusion（ MCAO） rabbits models were divided into three groups： cerebral infarction（ CI） group,stem cell group and saline group,and the last two groups were separately transplanted with h UCB-MSCs and saline. On 2 d,14 d after reperfusion,neurological deficits were evaluated by the neurological severity scores（ NSS） in the last three groups. The volume of cerebral infarction and the level of NAA,Cho,Cr,Lac in center area of ischemia were detected by using MR scanning.Results The neurological scores of h UCB-MSCs group significantly decreased,compared with CI group and saline group（ P 〉0. 05）. There was no significant difference between CI group and saline group（ P〈 0. 05）. Conclusion h UCB-MSCs treatment could increase the ratio of NAA/Cr,Cho/Cr,decrease the ratio of Lac/Cr,reduce the infarct volume,and could significantly improve the neurological function of rabbit.