三株大型真菌最佳培养条件的筛选

Screening of Optimal Culture Conditions of Three Strains of Macrofungi

以采自庞泉沟国家自然保护区的3种大型真菌(野蘑菇、罗曼尼蘑菇、梨形马勃)为试材,对子实体进行组织分离获得菌株,采用观察、测量、统计、分析等方法,研究比较了5种不同的碳源和氮源对3种菌株的菌丝生长速度的影响。结果表明:野蘑菇和罗曼尼蘑菇菌丝生长的最适碳源均为玉米粉,梨形马勃菌丝生长的最适碳源为葡萄糖;罗曼尼蘑菇和梨形马勃菌丝生长的最适氮源为硫酸铵,野蘑菇菌丝生长的最适氮源为硝酸铵。该研究为食用菌资源的开发利用奠定了基础。

Three species of macrofungi （ Agaricus arvensis , Agaricus romagnesii , L ycoperdon pyriforrne） from Pangquangou National Nature Reserve were used as the test materials, the strains from subsidiary entities was gotten by isolating tissue. The influence of five different carbon source and nitrogen source on the three strains of mycelium growth rate were researched by adopting such methods as observation, survey, statistics, analysis and so on. The results showed that the optimal carbon source of Agaricus arvensis and Agaricus romagnesii mycelium＇s growth was corn flour, the optimal carbon source of Lycoperdon pyriforme mycelium＇s growth was glucose; the optimal nitrogen source of Agaricus romagnesii and Lycoperdon pyriforrne mycelium＇s growth was ammonium sulfate;the optimum nitrogen source of Agaricus arvensis mycelium＇s growth was ammonium nitrate. The research laid the foundation for edible fungus resourcesr development and utilization.