复合酶法提取无患子总皂苷的工艺研究

Study of extraction of total saponins from sapindus with complex enzyme

无患子皂苷具有非常强的表面活性以及抗肿瘤、杀菌、消炎等生物活性.为探究复合酶法提取无患子总皂苷的最佳工艺条件,以总皂苷的提取率作为评价依据,通过单因素实验和正交实验进行探究,得出无患子总皂苷最佳的提取工艺条件为:复合酶质量浓度为0.6 mg/mL,酶解温度为50℃,pH值为4.5,酶解时间为2.5 h.在该条件下无患子总皂苷的提取率为64.40%.

The saponins of sapindus has strong surfactivity and biological activities such as antitumor, antiinflammatory and antimicrobial activities. In order to explore optimum extraction technology of the total saponin of soapnuts pectinase by complex enzyme method, it was determined through single factor and orthogonal experiment. The experimental results showed that optimum extraction technology conditions were as follows, the concentration of complex enzyme was 0.6 mg/mL, the temperature was 50 ℃, the pH was 4.5, the time was 2.5 hours. Under these conditions, average extraction ratio of total saponins was 64.40%.