摘要
目的研究白藜芦醇(RSV)防护糖尿病早期视损伤的分子机制。方法通过建立视网膜muller细胞(RMCs)体外高糖模型,采用免疫荧光双标、qRT-PCR、western blot、Annexin V/PI流式细胞技术检测高糖和RSV对微小RNA-29b(miR-29b)、特异蛋白1(SP1)表达及RMCs凋亡的影响。结果高糖培养后,miR-29b和SP1表达明显改变,RMCs凋亡率明显增加,RSV干预可明显抑制高糖诱导的miR-29b和SP1异常表达和RMCs凋亡;通过预先在RMCs中转染miR-29b类似物和miR-29b抑制剂研究发现,RSV抑制凋亡作用受明显影响(P<0.05)。结论 RSV通过调节miR-29b表达发挥抗糖尿病早期视损伤作用。
Objective To investigate the molecular mechanisms of protecting early diabetic retinopathy by resvertrol (RSV). Methods The effects of RSV on expressions of miRNA-29b (miR-29b) and specificity protein 1 ( SP1 ) as well as apoptosis of retinal muller ceils (RMCs) were investigated through development of rat RMCs hyperglucose model in vito, and mothods of immunofluores- cence,qRT-PCR,western blot and Annexin V/PI flow-cytometry. Results High glucose induced down-regulation of miR-29b and up- regulation of SP1 and increased the apoptosis of RMCs. The effects could be reversed by RSV in vitro. Moreover, the anti-apoptosis effect of RSV could be reversed by transfection of miR-29b analogues to RMCs and miR-29b inhibitor ( P 〈 0. 05 ). Conclusion Resveratrol can inhibit diabetic retinopathy in early stage by regulation of miR-29b expression.
作者
曾凯宏
王元
邓波
余雪梅
宋怡
周雪
黄璐娇
ZENG Kai-hong WANG Yuan DENG Bo YU Xue-mei SONG Yi ZHOU Xue HUANG Lu-jiao(Department of Clinical Nutrition ,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China School of Medicine, Chengdu University of Electronic Science and Technology , Chengdu 610054, China)
出处
《实用医院临床杂志》
2017年第4期22-26,共5页
Practical Journal of Clinical Medicine
基金
国家自然科学基金项目资助(编号:81202206)
四川省卫生计生委项目资助(编号:150216)