摘要
目的检测白念珠菌分泌的天冬氨酸蛋白酶6(Sap6)对人类单核细胞系(THP-1细胞)增殖的影响,明确Sap6蛋白是否具有诱导单核细胞凋亡的活性。方法用XTT比色法检测25、50μg/ml Sap6对THP-1细胞增殖率的影响;在流式细胞仪下用Annexin V-FITC/PI细胞凋亡试剂盒检测25、50μg/ml Sap6诱导THP-1细胞凋亡情况;用Con A-FITC/DAPI双染法在荧光显微镜下观察Sap6作用于THP-1细胞的位置。结果与25μg/ml比较,50μg/ml Sap6蛋白显著抑制人THP-1细胞增殖率(P=0.0001),并提高THP-1细胞早期凋亡率(P=0.0001);Sap6蛋白进入THP-1细胞内,结合于细胞核。结论白念珠菌分泌的Sap6在体外能够进入人单核细胞内,诱导凋亡,从而抑制细胞增殖率。
Objective To investigate the effects of secretory aspartic proteinase 6 (Sap6) from Candida albicans on human monocyte cell line ( THP-1 ) proliferation, and to find out whether Sap6 has an activity to induce the apoptosis of monocytes. Methods The effect of Sap6 on THP-1 cell proliferation was evaluated by XTF assay. Apoptotic rate of THP-1 cells induced by Sap6 was meas- ured by flow cytometry with Annexin V-FITC/PI apoptosis detection kit. The location of Sap6 on THP-1 ceils was examined by ConA- FITC/DAP1 staining under fluorescence microscope. Results 50 μg/ml of Sap6 had a significantly inhibitory effect on the proliferative rate of THP-1 ( P = 0. 0001 ). 50 μg/ml of Sap6 increased the early apoptotic rate of THP-1 ( P = O. 0001 ). Sap6 was internalized into THP-1 and combined with nucleus of the cells. Conclusion Secretory aspartic proteinase 6 from Candida albicans could access into human monocytes in vitro, and inhibit the proliferation of monocytes by inducing cell apoptosis.
作者
崔凡
李丹
陈霞
王有为
杨戈
CUI Fan LI Dan CHEN Xia WANG You-wei YANG Ge(Institute of Dermatology,Sichuan A- cademy of Medical Sciences & Sichuan Provincial People's Hospital,Chengdu 610031 ,China)
出处
《实用医院临床杂志》
2017年第4期30-32,共3页
Practical Journal of Clinical Medicine
基金
国家自然科学基金青年基金资助项目(编号:81101231)
四川省卫生和计划生育委员会科研基金资助项目(编号:150195)