高效液相色谱法测定蒙药制剂色布如-9中胡椒碱的含量

HPLC Determination of Piperine in Mongolian Medicine Preparation Se Bu Ru-9

目的:建立高效液相色谱法测定蒙药制剂色布如-9中胡椒碱的含量。方法:采用Diamonsi L C18(250 mm×4.6mm,5μm)色谱柱;以甲醇-水(77:23)为流动相;流速1.0 mL·min^(-1);检测波长343 nm;柱温为30℃。结果:胡椒碱在4~40μg·mL^(-1)(r=0.999 9)范围内线性关系良好,平均回收率为100.74%(n=9),RSD为0.18%。结论:本方法简便准确、稳定可靠,可用于蒙药制剂色布如-9的含量测定。

Objective：To establish？ a method for the determination of piperine in mongolian medicine preparation Se Bu Ru - 9. Methods： A DiamonsiL - C18 （250 mm × 4.6 mm, 5 μm） column was used with the mobile phase being chromatographic methanol -water（77：23 ）, flow rate being 1.0 mL · min -1 , detecting wavelength being 343 nm, the column temperature being 30℃. Results：The linear response range from 4 -40 μg · mL - 1 （ r = 0. 999 9, n = 6） of piperine ; the average recoveries （ n = 9） of the method was 100.74% with RSD 0. 18%. Conclusion：The method is simple, accurate, reproducible and stable, and can be used for the quality con- trol of mongolian medicine preparation Se Bu Ru -9.