PRRSV特异性肽对猪骨髓、脾脏、胸腺、肺门淋巴结中T细胞表型的影响

Effects of PRRSV specific peptides on T cell phenotype in porcine bone marrow,spleen,thymus and hilar lymph node

通过猪繁殖与呼吸障碍综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)特异性肽PRRSV-GP5(GP5aa96-104)、PRRSV-N(Naa49-57)刺激PRRS免疫后的仔猪骨髓、脾脏、胸腺、肺门淋巴结,用流式细胞仪检测T淋巴细胞表型的变化。结果显示:(1)骨髓和脾脏T细胞受到刺激时,首先转化为以CD4^+ T细胞为主,而胸腺和肺门淋巴结主要转化为以CD8^+ T细胞为主,并产生CD4^+ CD8^+ T细胞。其中,胸腺和肺门淋巴结中CD4的降低为一过性降低。(2)N特异性肽对骨髓CD3^+ 和CD4^+ CD8^+ T细胞有抑制作用,对脾脏和肺门淋巴结中的CD3^+ T细胞有促进作用,该抑制作用在48h后得以改善。(3)胸腺中T淋巴细胞转化能力最持久。(4)骨髓中T淋巴细胞开始以CD4^+ T细胞为主,后转为以CD8^+ T细胞为主。这为进一步研究有效防制PRRS的途径提供理论数据。

Porcine reproductive and respiratory syndrome causes serious damage to animal husband- ry in China. At present, there are some limitations and risks in PRRS inactivated vaccine and atten- uated vaccine. Therefore, it is very important to develop an effective PRRS subunit vaccine by studying the PRRS immune mechanism. PRRSV specific peptide PRRSV-GP5 （GPSaa96-104） and PRRSV-N （Naa49-57） were used to stimulate the bone marrow, spleen, thymus and hilar lymph nodes in piglets. When stimulated bone marrow and spleen T ceils, first transformed into CD4＋ T cells,while mainly transformed into CD8＋T cells in thymus and hilar lymph nodes and produced CD4＋ CD8＋T cells. The decrease of CD4 cells in thymus and hilar lymph nodes was a transient de- crease. N specific peptides have inhibitory effects on CD3＋T cells and CD4＋ CD8＋ T cells. And it promotes CD3＋ and CD4＋ CD8＋ T cells in the spleen and hilar lymph nodes. The inhibition was reduced after 48h. The ability of T lymphocyte transformation in thymus was the strongest. T lym phocytes in bone marrow first transformed into CD4＋ T cells,then turned into CD8＋T cells.