摘要
目的:建立蚁参护肝口服液中黄芪甲苷含量测定的方法。方法:采用高效液相色谱-蒸发光检测器,C_(18)(250mm×4.6mm,5μm)色谱柱;柱温:30℃;流动相为乙腈-水(40∶60);流速为1.0m L·min^(-1);蒸发光散射检测器气化温度:40℃。结果:黄芪甲苷在15.82μg/m L^79.10μg/m L范围内呈良好的线性关系,y=2.98x-28.17,R^2=0.9992。结论:该法准确、可靠、灵敏,可用于蚁参护肝口服液中黄芪甲苷的含量测定,为全面评价该药品的质量提供依据。
Objective :To establish the HPLC-ELSD method for determining the astragaloside Ⅳ in Yishen Hugan oral liquid. Methods : A column of C18 (250mm×4.6mm,5μm)was used. The ELSD was used as a detector, The column temperature was 30℃,the mobil phase consisted of acetonitrile-water (40:60) at a flow rate of 1.0mL·min-1,the gasification temperature of the ELSD was 40℃. Results: The linear range of astragaloside Ⅳ was 15.82μg/mL-79.10μg/mL (R2=0.9992),the average recovery was 98.7% (RSD=1.2%,n=6). Conclusions: The HPLC-ELSD method was sensitive, reproducible and accurate for the determination of Astragaloside Ⅳ in Yisheng Hugan Oral Liquid.
作者
翟晓茹
李永东
张红兵
张燕
Zhai Xiaoru Li Yongdong Zhang Hongbing Zhang Yan(China Institute for radiation protection,Shanxi Taiyuan, 03000)
出处
《北方药学》
2017年第7期1-2,共2页
Journal of North Pharmacy