PI3K-Akt-HIF-α信号通路在右美托咪定减轻大鼠肝缺血/再灌注致肺损伤中的作用

The role of PI3K-Akt-HIF-α signaling pathway in the protection of dexmedetomidine against limb liver ischemia/reperfusion(I/R)-induced acute lung injury in rats

目的:通过研究右美托咪定对大鼠肝缺血/再灌注致急性肺损伤中的作用及其可能作用机制。方法:建立大鼠肝缺血/再灌注肺损伤模型,以随机数字表法分为假手术组、肝缺血/再灌注组(I/R组)、I/R+右美托咪定组(D组)和I/R+右美托咪定组+wortmannin(DW组),通过ELISA和Western blot检测细胞炎症因子及PI3K-Akt-HIF-α信号传导通路蛋白的表达。结果:I/R组中MDA和MPO含量、细胞炎症因子表达及p-Akt和HIF-α蛋白含量较假手术组均明显升高;D组中上述肺损伤指标较I/R组均显著下调;与D组相比,DW组中MDA和MPO含量、细胞炎症因子表达及p-Akt和HIF-α蛋白含量均上调。

AIM： To investigate the role of PI3K-Akt-HIF-α signaling pathway in protection of dexmedetomidine against liver I/R- induced acute lung injury （ALI） in rats. Methods： 60 adult male SD rats were randomly divided into 4 groups （n = 15 each） using a random number table： sham operation group, I/R group, I/R ＋ dexmedetomidine group （D group） and ]JR ＋ dexmedetomidine ＋ wortmannin group （DW group）. ELISA and Western blot were used to explore the contents of the cytokines and the protein level of p- Akt as well as HIF-cdn peripheral blood and lung tissues. Results： Compared with sham operation group, the content of MDA and MPO, cytokine levels as well as the expression of p-Akt and HIF-α in I/R group were all increased markedly. However, the administration of dexmedetomidine attenuated the damage in the lung. Furthermore, compared with D group, the content of MDA and MPO, cytokine levels aS well as the expression of p-Akt and HIF-α were all up-regulated significantly in DW group. Conclusions： The PI3K-Akt-HIF-α signaling pathway was involved in the protective effect of dexmedetomidine against liver I/R-induced acute lung injury in rats.