HSPA2在胰腺癌中的表达及其临床意义

Expression and clinical significance of HSPA2 in pancreatic cancer

目的检测并对比热休克蛋白A2(HSPA2)在胰腺癌及癌旁胰腺组织中的表达水平,分析HSPA2蛋白在胰腺癌标本中的表达水平与其临床病理参数之间的相关性。方法采用实时定量PCR、Western blot法检测并对比胰腺癌及癌旁胰腺组织中HSPA2蛋白和mRNA的表达水平,采用免疫组织化学染色方法测定胰腺癌患者组织中HSPA2蛋白的表达,并探讨其表达与临床病理特征之间的关系。结果与癌旁胰腺组织相比,胰腺癌组织中HSPA2 mRNA和蛋白表达水平均明显升高。HSPA2蛋白在胰腺癌组织中的阳性表达率为68.8%(55/80)。HSPA2蛋白表达量与胰腺癌的分化程度、血管侵犯、TNM分期差异有统计学意义(P=0.011、0.005、0.012),而与患者性别、年龄、肿块大小及位置、血清CA199水平差异无统计学意义。结论 HSPA2在胰腺癌组织中的表达明显高于与之相应的癌旁胰腺组织,且HSPA2的高表达与胰腺癌的恶性进展、侵袭和转移等事件密切相关。

Objective To detect the expression of HSPA2 mRNA and protein in pancreatic cancer and correspond-ing adjacent nontumorous tissues, and investigate the expression of HSPA2 protein in pancreatic cancer and its as-sociation with clinicopathological characteristics. Methods Western blot and q PCR （real-time quantitative PCR ） was used to evaluate HSPA2 expression in pancreatic cancer and corresponding adjacent nontumorous tissues. Im- munohistochemistry was used to investigate the HSPA2 expression in pancreatic cancer and its association with clini-copathological characteristics. Results Both the mRNA and protein levels of HSPA2 were significant higher in pancreatic cancer tissues than those in their paired adjacent nontumorous tissues. High HSPA2 expression was de-tected in 68. 8% （55/80） of pancreatic cancer tissues. HSPA2 protein expression was significantly associated with tumor differentiation, vascular invasion, and status of metastasis （P = 0. O11,0. 005,0. 012） . Conclusion HS- PA2 expression is elevated in pancreatic cancer, and positively correlated the process of invasion, metastasis and progression of pancreatic cancer.