超声波辅助复合酶法优化提取香菇多糖

Optimum Technology on Extraction of Lentinan by Ultrasonic-assisted Composite Enzyme Method

利用超声波辅助纤维素酶与果胶酶复合法优化提取香菇多糖.方法:在单因素及正交实验基础上,以香菇多糖提取率为响应值,采用Box-Benhnken法设计响应面实验,得出香菇多糖最佳提取工艺参数.结果:香菇多糖最佳提取工艺:料液比1:21.17 g/m L、复合酶比1.96:1、温度48.21℃,在此条件下提取率最高为3.09%,与预测值3.14%相比,相对误差1.59%,模型拟合良好.结论 :与传统方法对比,超声辅助复合酶法明显提高香菇多糖得率,是较理想的提取方法.

Objective： ultrasonic -assisted composite enzymatic method for optimized extraction of Lentinan. method. Methods： based on single factor experiment and orthogonal experiment, choosed the yield of lentinan as the response value to designed the Box-Benhnken experiment. Results： the optimum conditions of extraction were solid-liquid ratio 1：21.17 g/mL, ratio of compound enzyme 1.96：1, temperature 48.21 ℃. On this condition, the lentinan yield was 3.09%, compared with the theoretical value （3.14%）, it has a smaller relative error at 1.59%, proving the regression model can well predict the actual situation. Conclusion： compared with the traditional method, ultrasonic -assisted enzymatic method increases the yield of lentinan, obviously, it was the ideal extraction methods.