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超声波辅助复合酶法优化提取香菇多糖 被引量:7

Optimum Technology on Extraction of Lentinan by Ultrasonic-assisted Composite Enzyme Method
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摘要 利用超声波辅助纤维素酶与果胶酶复合法优化提取香菇多糖.方法:在单因素及正交实验基础上,以香菇多糖提取率为响应值,采用Box-Benhnken法设计响应面实验,得出香菇多糖最佳提取工艺参数.结果:香菇多糖最佳提取工艺:料液比1:21.17 g/m L、复合酶比1.96:1、温度48.21℃,在此条件下提取率最高为3.09%,与预测值3.14%相比,相对误差1.59%,模型拟合良好.结论 :与传统方法对比,超声辅助复合酶法明显提高香菇多糖得率,是较理想的提取方法. Objective: ultrasonic -assisted composite enzymatic method for optimized extraction of Lentinan. method. Methods: based on single factor experiment and orthogonal experiment, choosed the yield of lentinan as the response value to designed the Box-Benhnken experiment. Results: the optimum conditions of extraction were solid-liquid ratio 1:21.17 g/mL, ratio of compound enzyme 1.96:1, temperature 48.21 ℃. On this condition, the lentinan yield was 3.09%, compared with the theoretical value (3.14%), it has a smaller relative error at 1.59%, proving the regression model can well predict the actual situation. Conclusion: compared with the traditional method, ultrasonic -assisted enzymatic method increases the yield of lentinan, obviously, it was the ideal extraction methods.
出处 《闽南师范大学学报(自然科学版)》 2017年第2期39-47,共9页 Journal of Minnan Normal University:Natural Science
基金 福建省大学生创新创业训练计划项目(闽教高[2013]65号)
关键词 超声波辅助 复合酶法 正交实验 响应面优化 ultrasonic assisted extraction method compound enzymatic method orthogonal design response surface optimization
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