对氧磷酶1的芳香酯酶活性检测新方法在冠心病中的应用

Application of the new method for the detection of the aromatic esterase activity of PON1 in coronary heart disease

目的观察对氧磷酶1(PON1)的芳香酯酶活性检测新方法在冠心病中的应用情况,分析PON1的芳香酯酶活性检测新方法的临床应用价值。方法选取2014年1月至2016年1月期间该院收治的冠心病患者86例作为研究对象,再选取同期来该院体检的健康人50例作为对照组,采用分光光度法测定冠心病患者和对照组研究对象的血清PON1芳香酯酶活性,同时统计不同病变程度冠心病患者的PON1芳香酯酶活性,对比冠心病患者和对照组研究对象以及不同病变程度的冠心病患者之间的PON1芳香酯酶活性,将冠心病患者的PON1芳香酯酶活性、年龄、体质量指数(BMI)、性别、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)以及三酰甘油(TG)列入多元线性回归方程中分析冠心病的影响因素,旨在通过PON1芳香酯酶活性判断冠心病患者的病情变化,为临床治疗提供一定的参考依据。结果冠心病患者与对照组的年龄、BMI、TG、TC以及LDH-C比较差异均具有统计学意义(P<0.05)。冠心病患者的PON1芳香酯酶活性明显低于对照组研究对象,差异具有统计学意义(P<0.05)。多支病变患者的PON1芳香酯酶活性明显低于单支病变患者,差异均具有统计学意义(P<0.05)。根据多元线性回归分析结果显示,冠心病与患者的年龄、性别、BMI、TC、LDL-C、TG以及PON1芳香酯酶活性均有关(P<0.05),其中与年龄、性别、BMI、TC、LDL-C以及TG呈正相关,与PON1芳香酯酶活性呈负相关。结论冠心病患者的PON1芳香酯酶活性会明显降低,且病变程度越重PON1芳香酯酶活性下降越明显,表明PON1芳香酯酶活性与冠心病呈负相关。

Objective To observe the effect of paraoxonase 1(PON1)application of the new method of arylesterase activity in patients with coronary heart disease,analysis of paraoxonase 1(PON1)of the clinical value of arylesterase activity in the new testing method.Methods From January 2014 to January 2016 in our hospital 86 patients with coronary heart disease as the research object,and then select the healthy people at the same time to the hospital physical examination of 50 as the control group to take the research object,spectrophotometric method for the determination of coronary heart disease patients and control subjects serum PON1 arylesterase activity,PON1 arylesterase activity at the same time with statistics the different degree of coronary heart disease,PON1 arylesterase activity between patients with coronary heart disease and control group comparison study and different severity of coronary heart disease patients,the patients with coronary heart disease PON1 aromatic ester enzyme activity,age,gender,BMI,TC,total cholesterol,low density lipoprotein cholesterol LDL-C and glycerin three greases TG included in the analysis of factors of coronary heart disease multiple linear regression equation,to determine the changes of patients with coronary artery disease by PON1 arylesterase activity,to provide a reference for clinical treatment.Results The activity of PON1 in patients with coronary heart disease was significantly lower than that of the control group,and the difference was statistically significant(P<0.05).Single branch lesions in patients with PON1 arylesterase activity was significantly higher than that of double vessel lesions and three lesions were double branch lesions in patients with PON1 arylesterase activity was significantly higher than that in three patients,the differences were statistically significant(P<0.05).According to the multiple linear regression analysis showed that coronary heart disease and the patient′s age,gender,BMI,TC,LDL-C,TG and PON1 arylesterase activity(P<0.05),which was related with age,gender,BMI,TC,LDL-C and TG were positively correlated,negatively correlated with PON1 arylesterase activity.Conclusion The PON1 activity of in patients with coronary heart disease is significantly decreased,and the extent of the disease is more severe,the more obvious the decline of PON1,the activity of PON1 shows a negative correlation with coronary heart disease.