大洋洲滨藜不定芽离体再生体系研究

Study on Adventitious Bud Regeneration System of Atriplex Nummularia Lindl

大洋洲滨藜是一种具有广泛应用价值的耐盐植物。为解决大洋洲滨藜快速繁殖问题,利用从澳大利亚引进的大洋洲滨藜茎段,建立了大洋洲滨藜的不定芽离体再生体系。研究了不同消毒方法、消毒时间、不同激素浓度对不定芽诱导和再生苗生根的影响;并经过靠苗将大洋洲滨藜再生幼苗成功移栽。结果表明:对大洋洲滨藜茎段的最适消毒方法为0.1%升汞(HgCl_2)消毒10 min,最适宜的不定芽诱导培养基为MS+2~3 mg·L^(-1)6-BA+0.1~0.2 mg·L^(-1)NAA+20 g·L^(-1)蔗糖+7 g·L^(-1)琼脂(pH 5.8),大洋洲滨藜再生苗最适宜的生根诱导培养基为1/2MS+0.3 mg·L^(-1)NAA+10 g·L^(-1)蔗糖+7 g·L^(-1)琼脂(pH 5.8)

Atriplex nummularia Lindl is an excellent salt-tolerant plant. In order to solve the problem of rapid propagation of A. nummularia,the adventitious bud regeneration system by using the stem section of A. nummularia was established. The effect of disinfection methods,disinfection time and hormone concentration on adventitious bud induction and rooting of regenerated shoots was studied. And the regenerated seedlings of A. nummularia were successfully transplanted. The results showed that the best method for disinfecting the stem section of was using HgCl_2 to sterilize for 10 min. The optimum medium for adventitious bud induction was MS ＋ 2 ~ 3 mg·L^（-1）6-BA ＋ 0. 1 ~ 0. 2 mg·L^（-1）NAA ＋ 20 g·L^（-1）sucrose ＋ 7 g·L^（-1）agar（ p H 5. 8）. And the optimal rooting medium was1/2MS ＋ 0. 3 mg·L^（-1）NAA ＋ 10 g·L^（-1）sucrose ＋ 7 g·L^（-1）agar（ pH 5. 8）.