肌注和口灌氟苯尼考在吉富罗非鱼体内的药代动力学

Study on the pharmacokinetics of florfenicol in tilapia

通过肌内注射、口灌两种给药方式,研究氟苯尼考在罗非鱼体内的药物代谢动力学特征。把吉富罗非鱼(GIFT Oreochromis niloticus)随机分成2组,控制水温在30℃,以15 mg/kg分别单剂量肌内注射、口灌给药。经高效液相色谱法(HPLC)测定血浆中氟苯尼考浓度,用Win Nonlin药动学软件分析药动学参数。结果表明:肌内注射氟苯尼考后,药物吸收较慢,消除较快,达峰时间(T_(max))=4 h,峰浓度(C_(max))=4.64μg/mL,消除半衰期(T_(1/2λ)z L)=10.45 h,药-时曲线下面积(AUC)=91.06μg·h/mL。口灌氟苯尼考后,药物吸收较快,消除较慢,T_(max)=1 h,C_(max)=5.92μg/mL,T_(1/2λ)z L=13.13 h,AUC=61.96μg·h/mL。肌内注射、口灌氟苯尼考后,二者的药动学参数差异显著,这一差异表明肌内注射给药吸收相对较慢,但更为完全(肌内注射氟苯尼考的AUC明显较大),消除相对较快。

To study the pharmacokinetics of florfenicol in, GIFT Oreochromis niloticus, after intramuscular （ i. m. ） and o-ral administrations, the fish were divided into two groups for i. m. and oral administrations. Florfenicol was administrated to tilapia at a single dose of 15 mg/kg body weight at 30℃.The concentration of florfenicol in the plasma was determined by HPLC method. Pharmacokinetic parameters were calculated by noncompartment modeling provided by WinNonlin 5. 12 soft-ware. The results show that the absorption of i. m. was slower while the elimination was faster, the time to peak plasma flor-fenicol concentration （ Tmax ） was 4 h , the maximum plasma concentration （ Cmax ） was 4.64μg/mL, the elimination half - life （T 1/2μL ） was 10. 45 h and the area under the concentration - time curve （ AUC） in plasma was 91. 0 6μg·h/mL. The absorption of oral administration was faster while the elimination was slower. The time to peak plasma florfenicol concentra-tion （Tmax） was 1 h, the Cmax was 5. 92μg·h/mL, T1/2μL was 13. 13 h, and the AUC was 61. 9 6μg·h/mL. It suggested that the absorption of oral administration was faster than that of i. m. while the elimination was slower, and the i. m. administration absorbed more completely.