HPLC-PDA同时测定不同品种及产地蜈蚣中8种核苷类成分

Simultaneous Determination of Eight Nucleosides in Scolopendra of Different Varieties and Origins by HPLC-PDA

目的:建立同时检测不同品种、不同产地蜈蚣样品中8种核苷类成分(胞嘧啶,胞苷,次黄嘌呤,黄嘌呤,尿苷,胸腺嘧啶,2'-脱氧肌苷和胸苷)含量的高效液相色谱法。方法:不同品种及产地蜈蚣提取物的分析选用Hedera ODS-2 C_(18)(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-水,梯度洗脱,流速1.0 m L·min^(-1),柱温30℃,检测波长254 nm,进样量5μL。结果:8种核苷类成分分离良好;在一定质量浓度内线性关系良好(r>0.999 9);加样回收率在95.73%~102.6%;11个批次蜈蚣样品中均含有8种核苷类成分,但含量存在差异。不同品种、不同产地的蜈蚣中核苷成分总量墨江蜈蚣含量最高,多棘蜈蚣其次,少棘巨蜈蚣和黑头蜈蚣中核苷类总成分比较接近。蜈蚣中次黄嘌呤和黄嘌呤明显高于其他核苷成分,少棘巨蜈蚣中次黄嘌呤的含量要高于其他品种。结论:该文所建立的方法快速简便,重复性、准确度良好,为蜈蚣的质量控制提供依据。

Objective： To establish a high performance liquid chromatography method for simultaneous determination of eight nucleosides in Scolopendra. Method： Hedera ODS-2 C18column （4.6 mm × 250 mm, 5 μm） was used for determination of eight nucleosides. The analyses were eluted with methanol and water in a gradient mode. The flow rate was 1.0 mL·min-1, and the column temperature was set at 30 ℃. The eight nucleosides were monitored and quantified at a wavelength of 254 nm, and the injection volume was 5 μL. Result： The investigated compounds, including cytosine, cytidine, hypoxanthine, xanthine, uridine, thymine, 2＇- deoxyinosine and thymidine, in the samples were well separated. The eight nucleosides showed a good linearity at a certain concentration （r 〉 0. 999 9）, and the mean recoveries of the eight nucleosides ranged from 95.73% to 102.6%. The contents of nucleosides in Scolopendra from different varieties varied significantly. The highest of content of the eight nucleosides was in Scolopendra mojiangica, and the second was in S. multidens. The content of the eight nucleosides in S. subspinipes mutilans and S. negrocapitis were close. The contents of hypoxanthine and xanthine in centipedes were significantly higher than those in other nucleosides, and the content of hypoxanthine inS. subspinipes mutilans was higher than that in other varieties. Conclusion： This high performance liquid chromatography method and quantitative analysis is simple, rapid, accurate and highly reproduced for simultaneous determination of eight nucleosides in Scolopendra, and helpful for its quality control.