长链非编码RNA HOXA末端转录本反义RNA和谷氨酸代谢型受体1在胰腺癌组织中的表达及其对患者预后的影响

Interrelation between expression of HOXA transcript at the distal tip and metabotropic glutamate receptor 1 and their influence on prognosis in pancreatic cancer patients

目的探讨长链非编码RNA（lcnRNA）HOXA末端转录本反义RNA（HOTFIP）和谷氨酸代谢型受体1（mGluR1）在胰腺癌组织中表达的临床意义及其对患者预后的影响。方法收集我院47例胰腺癌患者，反转录-聚合酶链反应（RT-PCR）法检测胰腺癌组织和癌旁组织中HOTTIP和mGluR1 mRNA的表达水平，Western blot法检测HOTTIP和mGluR1蛋白的表达，免疫组织化学法定量分析HOTTIP和mGluR1蛋白的表达，分析HOTTIP和mGluR1表达的临床意义、对患者预后的影响及其两者的相关性。结果胰腺癌组织中HOTTIP和mGlUR1 mRNA和蛋白的表达水平明显上调，与癌旁组织比较，差异有统计学意义（P=0．000）。在47例肿瘤样本中，32例（68．09％）样本中HOTFIP蛋白表达呈阳性，30例（63．83％）样本中mGlUR1蛋白呈阳性，其中对应的癌旁组织中HOTFIP和mGlUR1蛋白的阳性率分别是31．91％和36．17％，与癌旁组织比较，差异有统计学意义（P=0．000），并且HOTFIP和mGluR1的表达水平与胰腺癌的淋巴结转移、分化程度和TNM分期有关（P值分别为0．000、0．004、0．000）。HOTFIP和mGluR1表达呈阳性的患者生存时间明显低于阴性患者，两者差异有统计学意义（P=0．029、0．047）。Cox比例风险模型多因素分析结果表明，淋巴结转移、分化程度、TNM分期、HOTTIP和mGlUR1表达水平是影响胰腺癌患者预后的独立危险因素（P值分别为0．022、0．048、0．026、0．000、0．002）。HOTFIP和mGluR1在胰腺癌患者中表达呈明显正相关（r=0．689，P=0．002）。结论HOTTIP和mGluR1的高表达与胰腺癌的发生、发展和预后有关。

Objective To investigate the clinical significance and interrelation of long - chain non -coding RNA （lcnRNA） HOXA transcript at the distal tip （HOTFIP） and metabotropic glutamate receptor 1 （mGluR1） in pancreatic cancer patients. Methods Pancreatic cancer samples, which were trea- ted in our hospital were collected. The expression levels of HOTTIP and mGlUR1 mRNA were detected by reverse transcriptase - polymerase chain reaction （ RT - PCR） in fresh pancreatic cancer tissue, and those of HOTTIP and mGluR1 protein by Western blotting and immunohistoehemistry. Moreover, the relationship between the expression of HOTTIP, mGlUR1 and clinicopathological features was analyzed. Results The levels of HOTTIP and mGlUR1 mRNA and protein were significantly up - regulated in pancreatic cancer tissues as compared with normal pancreatic tissue （ P = 0. 000 ）. The expression of HOTTIP protein was positive in 32 samples （68.09%） and mGlUR1 protein was positive in 30 samples （63.83%）. The positive rate of HOTTIP and mGlUR1 in adjacent tissues was 31.91% and 36. 17% , respectively, and the difference was also statistically significant （ P = 0. 000 ）. The expression of HOTTIP and mGluR1 was cor- related with lymph node metastasis, differentiation degree and TNM stage （ P = 0. 000, 0. 004, 0. 000）. The survival time of HOTTIP and mGlUR1 positive patients was significantly shorter than that of negative patients （P = 0. 029, 0. 047）. Cox regression multivariate analysis showed that lymph node metastasis,differentiation degree, TNM stage, HOTTIP and mGlUR1 expression were independent prognostic factors for pancreatic cancer （P = 0. 022, 0. 048, 0. 026, 0. 000, 0. 002）. In addition, HOTTIP and mGlUR1 expression showed a significant positive correlation in cervical cancer patients （ r = 0. 689, P = 0. 002 ）. Conclusion The positive expression of HOTTIP and mGlUR1 is associated with the occurrence, progression and prognosis of pancreatic cancer.