磷酸肌醇3激酶／蛋白激酶B信号通路在膀胱癌中的表达及与膀胱癌干细胞的关系

Expression of phosphatidylinositol 3 kinase/protein cells and its relationship with cancer stem cells Xu Dongliang kinase B signaling pathway in bladder cancer

目的研究磷酸肌醇3激酶（PI3K）／蛋白激酶B（Akt）信号通路在人类膀胱癌中的表达情况，并观察其与膀胱癌干细胞的关系，探讨PI3K／Akt信号通路住膀胱癌发生发展中的意义。方法采用反转录-聚合酶链反应（RT—PCR）和Western blot检测人膀胱癌中PI3K、磷酸化Akt（p-Akt）蛋白及mRNA的表达；采用免疫荧光双标技术检测膀胱癌干细胞标志物CD133存膀胱癌组织中的表达及其与p-Akt的共表达。结果在膀胱癌组织中，PI3K、p-Akt蛋白（PI3K：1．05±0．03，p-Akt：1．21±0．04）及PI3K mRNA（0．68±0．03）水平表达明显高于正常膀胱组织（PI3K蛋白：0．68±0．04，p-Ak蛋白：0．71±0．02，PI3K mRNA：0．42±0．02），膀胱癌下细胞标志物CD133在膀胱痛组织中有表达；p-Akt与CD133的共表达呈阳性。结论PI3K／Akt信号通路存膀胱癌组织中被激活，并且p-Akt与膀胱癌组织中膀胱癌干细胞标志物CD133存在共表达，推测通过干扰PI3K／Akt信号通路抑制膀胱癌干细胞异常分化可防治膀胱痛的发生发展。

Objective To examine the expression of phosphatidylinositol 3 kinase （PI3K）/protein kinase B （Akt） signaling pathway in hladder cancer cells, and demonstrate relationship with cancer stein cells. Methods The expression of PI3K, phosphorylated protein kinase B （ p - Akt） in bladder cancer cells was detected by reverse transcription -polymeric chain reaetion（ RT- PCR） and Western blotting. Double immunoflnorescence analysis was applied to detect the expression of CD133, the Co- expression of p - Akt and CD133. CD133 is one of surface marker of bladder cancer stem ceils. Results We detected a strong up - regulation of PI3K, p - Akt （ PI3K protein ： 1.05 ± 0. 03, p - Akt protein ： 1.21 ±0. 04, PI3K mRNA： 0. 68 ± 0. 03 ） in bladder cancer tissue samples compared to norlnal bladder tissues （PI3K protein： 0.68 ±0.04, p - Akt protein： 0. 71 ±0. 02, PI3K mRNA： 0. 42 ±0. 02） by Western blot and RT - PCR approaches. We also detected a strong Co - expression of p - Akt and CD133 in bladder cancer tissue sampies. Conclusion The expression of PI3K/Akt signaling pathway is up - regulation in bladder cancer tissues, and the expression of CD133 and p- Akt was co- expressed, suggesting that the activation and differentiation of bladder eaneer stem cells eould be inhibited by interference with PI3K/Akt signaling pathway to prevent the oecun＇ence and development of bladder cancer.