放射工作人员周围血GADD45 mRNA表达与受照剂量关系

Correlation between GADD45 mRNA expression and exposure dose in peripheral blood of radiation workers

目的分析生长抑制和DNA损伤基因45(GADD45)mRNA在放射工作人员周围血中的表达情况及其与受照剂量的关系。方法采用典型抽样方法,以广东省某医院97名放射工作人员为放射组,以不接触电离辐射及其他职业性有害因素的48名医院行政工作人员为对照组,采用实时定量聚合酶链式反应法检测2组人员周围血中GADD45 mRNA表达水平。采用热释光剂量法检测和收集放射组人员过去1年的个人剂量。结果放射组人员周围血GADD45 mRNA相对表达水平高于对照组(P<0.01)。放射组人员个人剂量、性别、年龄、工龄、吸烟、饮酒等因素均与GADD45 mRNA相对表达水平无相关关系(P>0.05)。结论电离辐射可导致放射工作人员周围血中GADD45 mRNA表达上调,但GADD45 mRNA表达与其个人剂量无线性关系。

Objective To analyze the growth arrest and DNA damage gene 45 （GADD45） mRNA expression in peripheral blood of radiation workers, and its relationship with exposure dose.Methods Using a typical sampling method, 97 radiation workers from a hospital of Guangdong were selected as radiation group, 48 hospital administrative staff without exposure to radiation and other occupational hazard factors were selected as control group.The real-time quantitative polymerase chain reaction was used to detect GADD45 mRNA expression of two groups.With the use of thermoluminescence dosimetry, we detected and collected personal dose data of radiation group over the past year.Results The GADD45 mRNA relative expression of the radiation group was higher than that of the control group （P〈0.01）.There was no correlation between GADD45 mRNA relative expression and factors such as personal dose, sex, age, length of service, smoking, and alcohol drinking （P〉0.05）.Conclusion The ionizing radiation can lead to up-regulation of GADD45 mRNA expression in peripheral blood of radiation workers, but there was no linear relationship between GADD45 mRNA expression and its personal dose.