不同类型射线对人淋巴细胞染色体畸变影响

Effects of different radiation on chromosome aberration in human lymphoblastoid cells

目的比较铁-56(^(56)Fe^(17+))、碳-12(^(12)C^(6+))重离子束与钴-60(^(60)Co)γ射线对人淋巴细胞染色体畸变的影响。方法将人淋巴细胞按照射剂量分为0.1、0.3、0.5、0.7、1.0、2.0 Gy照射组和0.0 Gy对照组,分别采用^(56)Fe^(17+)(传能线密度为400.0 ke V/μm)、^(12)C^(6+)(传能线密度为26.0 ke V/μm)重离子束和^(60)Coγ射线进行照射,照后48 h收获细胞,采用光学显微镜人工计数法检测人淋巴细胞染色体双着丝粒体加着丝粒环(以下简称"双+环")畸变率和畸变细胞率。结果人淋巴细胞经^(56)Fe^(17+)、^(12)C^(6+)重离子束和^(60)Co γ射线照射后,0.3~2.0 Gy照射组^(12)C^(6+)重离子束诱导的"双+环"畸变率均高于相同剂量组的^(56)Fe^(17+)重离子束和^(60)Co γ射线(P<0.05),0.1~2.0 Gy照射组^(12)C^(6+)重离子束诱导的畸变细胞率均高于相同剂量组的^(56)Fe^(17+)重离子束和^(60)Coγ射线(P<0.05)。3种射线诱导的人淋巴细胞畸变效应在0.0~2.0 Gy内均呈随剂量增加而升高的趋势(P<0.01)。^(56)Fe^(17+)重离子束的相对生物效应值低于相同剂量的^(12)C^(6+)重离子束。结论^(12)C^(6+)重离子束导致的人淋巴细胞染色体畸变较^(60)Coγ射线和^(56)Fe^(17+)重离子束严重。

bjective To compare the effects of 56Fe17＋, 12C6＋ ion beams and 60Co γ rays on chromosome aberration in human lymphoblastoid cells.Methods The human lymphoblastoid cells were divided into 0.1, 0.3, 0.5, 0.7, 1.0, 2.0 Gy irradiated groups and 0.0 Gy control group.They were separately exposed to 56Fe17＋ ion beams （linear energy transfer was 400.0 keV/μm）, 12C6＋ ion beams （linear energy transfer was 26.0 keV/μm） and 60Co γ rays.Chromosome specimens were harvested 48 hours after irradiation.The effects of different radiation on dicentric plus centric ring （＂d＋r＂） aberration rate and chromosome aberration in human lymphoblastoid cells were detected by light microscope with artificial counting.Results The ＂d＋r＂ aberration rates induced by 0.3-2.0 Gy 12C6＋ ion beams were significantly higher than those of 56Fe17＋ ion beams and 60Co γ rays at the same dose （P〈0.017）.Chromosome aberration cell rates of 0.1-2.0 Gy 12C6＋ ion beams were significantly higher than those of 56Fe17＋ ion beams and 60Co γ rays at the same dose （P〈0.017）.At the dose range of 0.0-2.0 Gy, chromosome aberration effects of three kinds of radiations were gradually increased （P〈0.01）.The relative biological effectiveness of 56Fe17＋ ion beams was lower than that of 12C6＋ ion beams.Conclusion The chromosome aberration induced by 12C6＋ ion beams was more serious than that of 60Co γ rays and 56Fe17＋ ion beams.