摘要
为了研究大鲵蜕皮中具有抗菌活性的小分子肽,采用SDS-PAGE电泳和SephadexG-75凝胶层析分离分析大鲵蜕皮中的成分,并采用氯化三苯四氮唑微量稀释法检测了抑菌活性。结果表明:大鲵蜕皮含有一种分子量约为15 000的小分子肽,该物质对5种试验菌种均有一定抑制活性。大鲵蜕皮发现的小分子肽可能是一种新的抑菌肽,其可能为大鲵人工养殖创造出新的产业价值。
To study the antimicrobial activity of small molecules in appearance of this peptide from molting of the Andrias davidianus. Methods: using SDS-PAGE electrophoresis and gel chroma- tography separation SephadexG - 75 analysis of appearance of this composition, and USES the three benzene chloride four azole nitrogen trace dilution method to detect antibacterial activity. Andriasdavidianus contains a small molecular peptide with molecular weight of about 15 000, and the small molecular peptide has certain inhibitory activity against five kinds of experimental strains . This small molecular peptide has promise to be a new kind of antibacterial peptide.
作者
王茁
WANG Zhuo(College of Life Science and Food Engineering, Shaanxi Xueqian Normal University, Xilan 710100, Shaanxi, China)
出处
《陕西师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2017年第4期87-89,共3页
Journal of Shaanxi Normal University:Natural Science Edition
基金
陕西省科技计划项目(2013JM3017)
陕西省教育厅项目(2013JK0740)
陕西学前师范学院院级科研项目(2014QNKJ076)
关键词
大鲵
大鲵蜕皮
SDS-PAGE电泳
抗菌活性
Andrias davidianus
andrias molting~ SDS-PAGE electrophoresis
antimicrobial activity