摘要
目的:研究翼核果素对人肝癌Hep G2细胞增殖和凋亡的影响,并探讨其可能的机制。方法:以不同浓度的翼核果素作用于体外培养的Hep G2细胞,采用MTT法检测细胞生长抑制率,HE、吖啶橙染色观察细胞形态,流式细胞仪检测细胞凋亡率和周期,激光共聚焦显微镜观察细胞骨架蛋白F-actin形态学变化。结果:翼核果素可明显抑制人肝癌Hep G2细胞的增殖且呈剂量依赖性,作用于Hep G2细胞24 h的IC50值为124.77μmol·L^(-1)。显微镜可见细胞核变形、染色体聚集等典型的凋亡形态学改变。流式细胞仪检测结果显示,60、90μmol·L^(-1)翼核果素给药组细胞凋亡的百分率显著高于对照组,G2/M期细胞比例显著高于对照组,细胞骨架蛋白F-actin呈现解聚断裂状态。结论:翼核果素可抑制Hep G2细胞的增殖,诱导细胞凋亡,其机制可能与细胞周期阻滞、细胞骨架蛋白F-actin解聚有关。
Objective: To study the inhibition effects of ventilagolin and proliferation and apoptosis on human hepatocellular carcinoma cells HepG2, discusses the possible mechanisms. Methods: HepG2 cells in culture medium were treated with different concentrations of ventilagolin. The inhibitory rate of the ceils was measured by MTT assay. The morphology of cells was observed by HE and Acridine orange stain. Cell cycle changes and apoptotic rate were detected by Flow Cytometry (FCM). The mophological changes of cytoskeletal protein F-actin were observed by laser confocal microscopy. Results: The growth of HepG2 cells was significantly inhibited by ventilagolin, and the growth inhibitory rate increased with increasing concentration. IC50 of ventilagolin was 124. 77 μmol. L^- 1 after 24 hours. A characteristic apoptosis change including nuclear disintergration and chromatin agglomerate was displayed. The apoptosis rate of HepG2 cell treated with 60, 90 μmol· L^-1 ventilagolin was significantly higher than that of the control group, and the percentage of G2/M phase of I-IepG2 cell was also significantly higher than that of the control group. F-actin eytoskeletal protein was showed from polymerization to depolymerization after ventilagolin treatment. Conclusion: Ventilagolin can obviously inhibit the proliferation and induce apoptosis of HepG2 cells. The mechanisms may be due to blocking cell cycle and depolymerizating cytoskeletal protein F-actin.
作者
刘瑛
陆国寿
卢文杰
王丽
黄周锋
胡筱希
LIU Ying LU Guo-shoua LU Wen-jie WANG Li HUANG Zhou-feng HU Xiao-xi(Guangxi Key Laboratory of Traditional Chinese Medicaine Quality Stardands Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning 530022, Chin)
出处
《中国中医基础医学杂志》
CSCD
北大核心
2017年第6期791-794,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
广西自然科学基金系项目(2016GXNSFAA380092)-瑶药紫九牛中翼核果素衍生物的合成及抗肿瘤活性研究
广西中药质量标准研究重点实验室研究课题(桂中重自201601)-瑶药紫九牛中翼核果醌-I的初步结构修饰与活性研究
广西中药质量标准研究重点实验室青年骨干创新基金(桂中重自201506)-瑶药翼核果化学成分及活性的系统性研究