红花黄色素对大鼠急性脑缺血后不同时间点炎性损伤的保护作用

Protective effect of safflower yellow pigment on inflammatory injury in rats in the different time points after acute cerebral ischemia

目的:初步探索红花黄色素(以下简称SY)对大鼠急性脑缺血后不同时间点炎性损伤的保护作用。方法:健康雄性SD大鼠随机分假手术组、MCAO模型组和SY组。假手术组、模型组和SY组又各分4个时间点(3h,6h,12h,24h)。即假手术组(3h,6h,12h,24h),MCAO模型组(3h,6h,12h,24h),SY组(3h,6h,12h,24h)共12组各15只。术后取眶周血用ELISA法测炎症因子TNF-α、IL-6及IL-10水平,之后断头取脑切片行HE染色分析。结果:SY可抑制脑缺血后促炎因子TNF-α(3 h、24 h)和IL-6(3 h、6 h和24 h)升高的作用,并且促进抗炎因子IL-10含量升高(3 h、12 h和24 h)。SY对大鼠急性脑缺血后24h脑组织切片炎性损伤有明显的缓解作用。结论:SY对大鼠急性脑缺血后24小时炎性损伤有显著的保护作用。

Objective： To explore protective effect of safflower yellow pigment （SY） on inflammatory injury in rats in the different time points after acute cerebral ischemia.Methods： One hundred and eighty healthy male SD rats were randomized divided into 12 groups, including sham groups （3 h, 6 h, 12 h, 24 h） , MCAO groups （3h, 6h, 12h, 24h） , SYgroups （3h, 6h, 12h, 24h） （n=15ineachgroup） .TNF-α, IL-6 and IL-10 levels in rats serum were measured by ELISA .The pathologic morphology in rats brains were observed using HE staining analysis.Results： After being treated with SY, the increased levels of TNF- α （3 h, 24 h） and IL - 6 （3 h, 6 h and 24 h） were significantly inhibted, while IL - 10 （3 h, 12 h and 24 h） were significantly promoted. In addition, the rats brains inflammatory injury in 24th hour after acute cerebral ischemia were obviously relieved.Conclusion： SY can relieve the inflammatory injury in rats brains in the 24th hour after acute cerebral ischemia.