人肾脏促红细胞生成素cDNA基因的克隆

The clone of EPO cDNA gene from human kidney

目的:克隆促红细胞生成素(erythropoietin,EPO)c DNA基因,为重组EPO的制备奠定基础。方法:从人肾脏中提取总RNA,采用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)法扩增EPO c DNA基因后,将其插入到克隆载体p JET 1.2中,DNA测序法鉴定基因序列。结果:对克隆到的EPO c DNA基因进行DNA测序分析鉴定,其结果与Gene Bank中序列完全一致。结论:成功从人肾脏中克隆扩增了EPO c DNA基因,为重组EPO的制备奠定了基础。

Objective：To lay a foundation for the preparation of recombinant erythropoietin （EPO） by cloning EPO cDNA gene. Methods： Total RNA was extracted from human kidney and the reverse transcription - polymerase chain reaction （ RT - PCR） method was adopted to amplify EPO cDNA gene, which was then inserted into a cloning vector pJET 1.2. DNA sequencing method was used to identify gene sequence. Results： The cloned EPO cDNA gene sequence was in accord with sequence in GeneBank through DNA sequencing analysis. Conclusion： EPO cDNA gene from human kidney is successfully cloned and lays a foundation for the preparation of recombinant EPO.