碳源饥饿对癌细胞和正常细胞增殖的影响

Effects of glucose deprivation of HeLa cells and human umbilical cord cells

研究了共培养条件下碳源饥饿对癌细胞和正常细胞形态、生长和凋亡的影响.采用细胞体外共培养的方法,实验分4组:对照组(25 mmol/L葡萄糖)及5,1和0 mmol/L葡萄糖组,分别处理共培养条件下的HeLa癌细胞和人脐带正常细胞,在倒置显微镜下观察细胞的形态与生长,吖啶橙/溴乙锭(AO/EB)双重荧光染色检测凋亡细胞的形态及数量.结果表明:对照组HeLa癌细胞和人脐带正常细胞都分布均匀,贴壁性好,形态饱满,多呈规则的梭形或多角形,折光性好,AO/EB双染绿色荧光分布均匀;实验组糖浓度越低,细胞存活时间越短,细胞收缩变圆并逐渐凋亡,AO/EB双染细胞由绿色荧光逐渐变为橙黄色荧光;相同糖浓度条件下人脐带正常细胞比HeLa癌细胞存活时间更长.说明碳源饥饿使癌细胞先于正常细胞死亡.

To investigate the influence of different glucose and apoptosis of HeLa cell which were directly co-cultured concentration medium on the morphology, growth with human umbilical cord cells. A control group and three experimental groups （ 5 mmol/L, 1 mmol/L, 0 mmol/L ） were designed. After co-cultured for 24, 48, 72 h, cells were observed and took pictures under the inverted microscope and stained with AO/EB were observed under fluoroscope. The results under the inverted microscope ： cells population of control group were sufficient, presented irregular and adherent growth, cone-shaped or polygonal cell body, its boundary was clear and obvious halo, and highly refractive. It was found that the numbers of cells increased gradually with the increment of glucose concentration. Cells stained with AO/EB were seen dark green or emit green. In the experimental group, the lower the glucose concentration was, the shorter the cell survival time was. Cells stained with AO/EB were gradually changed from green to orange yellow fluorescence. The same glucose concentration in human umbilical cord cells survived longer than HeLa cells. Therefore, the effect of carbon starvation on HeLa cell proliferation inhibition and induced apoptosis was more obvious than that of human umbilical cord cells. HeLa cells were more dependent on glucose than human umbilical cord cells.