大麦皮壳率快速测定方法研究

Study on Fast Method for Determination of Seed Husk Content in Barley

大麦皮壳率测定主要有氨水浸泡法和千粒重量法,但均不适合进行快速、高通量样品分析。纤维素是大麦皮壳的主要化学成分。采用新型纤维素溶解体系,探讨了快速、稳定和高通量测定大麦皮壳率的方法。结果表明,NaOH溶液、NaClO溶液、NaOH/尿素体系都能用于测定大麦皮壳率,但碱性次氯酸钠法效率更高。通过比较NaOH浓度、NaClO浓度以及处理时间对大麦皮壳率测定的影响,得出4% NaOH配合20%的NaClO溶液,经过2min的短沸是一个比较快速稳定的方案。比较碱性次氯酸钠法与氨水浸泡法、千粒重量法、NaOH/尿素法的准确性与重复性,发现碱性次氯酸钠法准确度高、重复性好、效率高,最适合作为快速、稳定、大批量测定大麦皮壳率的方法。

Aqueous ammonia method(SAA)and sulphuric acid method for soaking were two main methods for determination of barley seed husk content.However,the two methods were not suitable for measure large numbers of samples.Cellulose was the major chemical component of barley husk.The new cellulose dissolution system was used to develop a rapid,stable and high-throughput method for barley husk content determination.The results confirmed that alkaline sodium hypochlorite was more effective compared to sodium hydroxide treatment,sodium hypochlorite solution and sodium hydrate/urea aqueous solution for barley husk content determination.The concentrations of NaOH,NaClO,and the treatment time were adjusted to produce a stable and fast alkaline sodium hypochlorite method.The results showed that 4% NaOH combined with 20% NaClO and treated by2 min boiling was the most reliable combination of alkaline sodium hypochlorite method.The accuracy and repeatability of alkaline sodium hypochlorite method were compared with the SAA and sulphuric acid method,which further confirms that the alkaline sodium hypochlorite method was the most suitable method for determining large number of barley husk contents.