费菜总黄酮分离纯化方法研究

Study on the Separation and Purification of Total Flavonoids from Sedum aizoon L.

[目的]开发高纯度费菜总黄酮的分离纯化工艺。[方法]以大孔树脂为载体,对上样量及层析条件进行优化,再进一步采用结晶的方法进行纯化。[结果]选用聚合物纳米微球PS RPC-300作为最佳层析填料,洗脱速度为5 m L/min,洗脱剂为70%乙醇,上样量为5.0 g/kg填料,结晶溶剂为丙酮;在此优化条件下所得产品的纯度为95.1%。[结论]此分离纯化方法简便可靠,分离效果好。

[ Objective ] The research aimed to develop the separation and purification processes of high-purity total flavonoids from Sedum aizoon L. [ Method] With the macroporous resin as the carrier, the loading volume and the chromatographic conditions were optimized, and then further purified by crystallization method. [ Result] Type of PS RPC -300 nanoscale polymer particles was selected as the best chromatography filler. The optimal conditions were as follows：the elution speed was 5.0 mL/min,70% ethanol was used as the eluant,the amount of sample was 5 g/kg filter, and acetone was used as the crystallization solvent. The purity of the final product was 95.1% under the optimal conditions. [ Conclusion ] The separation and purification method is simple and reliable, and the separation effect is good.