消岩汤对肺腺癌A549实体荷瘤小鼠肿瘤细胞凋亡干预机制的研究

Research on intervention mechanism of Xiaoyan Decoction on apoptosis of lung adenocarcinoma A549 cells in tumor-burdened mice

目的分析消岩汤干预下Survivin siRNA对肺腺癌A549实体荷瘤小鼠肿瘤细胞凋亡的影响。方法以人肺腺癌A549实体移植瘤裸鼠模型为研究对象,将消岩汤含药血清及siRNA转染后的A549细胞分别进行瘤内注射,通过流式细胞术观察小鼠体内细胞凋亡情况;免疫细胞化学法检测Survivin蛋白表达。结果 siRNA转染组对Survivin蛋白表达的抑制率明显高于消岩汤组,但显著低于消岩汤+siRNA转染组。siRNA转染组和消岩汤组均可诱导细胞凋亡,且siRNA转染组的诱导作用强于消岩汤组,消岩汤+siRNA转染组可协同增强诱导细胞凋亡作用。结论消岩汤联合Survivin siRNA可协同增强诱导细胞凋亡作用。

Objective To analyze the elimination of Xiaoyan Decoction intervened survivin siRNA in lung adenocarcinoma A549 cells tumor-burdened mice tumor cell apoptosis. Methods Human lung adenocarcinoma A549 entity nude mouse transplantation tumor model was studied as the research object. Xiaoyan Decoction medicated serum and siRNA cells after transfection group was injected within the tumor respectively by flow cytometry to observe apoptosis and cell cycle distribution in mice; Survivin protein expression was detected by immunocytochemistry method. Results Transfection group＇s inhibition of the expression of survivin protein was obviously higher than that of Xiaoyan Decoction group, but significantly lower than Xiaoyan Decoction and siRNA group. Transfection group and Xiaoyan Decoction both could induce cell apoptosis, and induction effect of transfection group was stronger than Xiaoyan Decoction group, Xiaoyan Decoction combined with transfection group could ernhance the induction of cell apoptosis. Conclusion Xiaoyan Decoction combined with Survivin apoptosis induced by siRNA can synergy to enhance its role.