大鼠ig毛蕊异黄酮-7-O-β-D-葡萄糖苷后尿中代谢产物的分离和鉴定

Isolation and identification of metabolites in rat urine after ig administration of calycosin-7-O-β-D-glucopyranoside

目的研究大鼠ig毛蕊异黄酮-7-O-β-D-葡萄糖苷后尿中代谢产物。方法采用制备液相进行分离和纯化,通过核磁和质谱数据确定化合物的结构。结果从大鼠ig毛蕊异黄酮-7-O-β-D-葡萄糖苷后的尿中提取分离得到5个代谢产物,分别为毛蕊异黄酮(M_1)、3′,4′,7-三羟基异黄酮(M_2)、大豆素(M_3)、毛蕊异黄酮-3′-O-β-D-葡萄糖醛酸苷(M_4)、毛蕊异黄酮-3′-O-β-D-葡萄糖醛酸甲酯苷(M_5)。结论代谢产物M_5为新的化合物。

Objective To study the metabolites from rats urine after ig administration of calycosin-7-O-β-D-glucopyranoside. Methods The constituents were isolated and purified by preparative liquid chromatographic technique, and the structures were identified by spectroscopic analyses including ESI-MS, NMR, and 2D-NMR. Results Five metabolites were isolated from rats urine after ig administration of calycosin-7-O-β-D-glucopyranoside. They were identified as calycosin（M1）, 3′,4′,7-trihydroxyisoflavone（M2）, daidzein（M3）, calycosin-3′-O-β-D-glucuronide（M4）, and calycosin-3′-O-β-D-glucuronide methyl ester（M5）. Conclusion M5 is a new compound.