发酵豆渣中低聚糖对益生菌增殖的研究

Study on proliferation of oligosaccharides in fermented soybean residue

目的:研究粗壮脉纹孢菌发酵豆渣中低聚糖对植物乳杆菌NCU116和青春双歧杆菌CICC 6178增殖的影响。方法:将植物乳杆菌NCU116和青春双歧杆菌CICC 6178分别培养48 h,测定生长曲线、最大比生长速率、延迟期、发酵液p H曲线和基质消耗率。结果:2 g/L发酵豆渣低聚糖组的植物乳杆菌NCU116发酵液最大吸光度、p H减小值、基质消耗率(p<0.05)均小于2 g/L菊糖组(阳性对照组),2 g/L发酵豆渣低聚糖组的青春双歧杆菌CICC 6178最大吸光度、p H减小值、基质消耗率(p<0.05)均大于2 g/L菊糖组,所有发酵豆渣低聚糖组对两种益生菌的最大比生长速率均大于2 g/L菊糖组,2、6 g/L发酵豆渣低聚糖组的植物乳杆菌NCU116延迟期小于2 g/L菊糖组,各发酵豆渣低聚糖组的青春双歧杆菌CICC 6178延迟期大于2 g/L菊糖组。结论:粗壮脉纹孢菌发酵豆渣中低聚糖对植物乳杆菌NCU116的增殖作用不如菊糖,对青春双歧杆菌CICC 6178的增殖作用优于菊糖。

Objective： To investigate the effects of oligosaccharides on the proliferation of Lactobacillus plantarum NCU116 and Bifidobacterium adolescentis CICC 6178 in soybean residue fermented by Neurospora crassa. Methods： The growth curve,maximum growth rates,lag parameters,p H value of fermentation broth and substrate consumption rate were determined by incubating Lactobacillus plantarum NCU116 and Bifidobacterium adolescentis CICC 6178 for 48 h.Results： The maximal absorbance,p H reduction value and substrate consumption rate of the fermentation broth of Lactobacillus plantarum NCU116 of2 g/L fermented soybean residue oligosaccharides group were lower than those of 2 g/L inulin group（ positive control group）.The maximal absorbance,p H reduction value and substrate consumption rate of the fermentation broth of Bifidobacterium adolescentis CICC 6178 of 2 g/L fermented soybean residue oligosaccharides group were higher than those of 2 g/L inulin group.The maximum growth rates of all fermented soybean residue oligosaccharides groups to the two probiotics were greater than those of 2 g/L inulin group,the lag parameters of Lactobacillus plantarum NCU116 of 2,6 g/L fermented soybean residue oligosaccharides groups were less than those of 2 g/L inulin group. The lag parameters of Bifidobacterium adolescentis CICC6178 of all fermented soybean residue oligosaccharides groups were more than 2 g/L inulin group.Conclusion： The proliferation effect of Lactobacillus plantarum NCU116 of oligosaccharides in soybean residue fermented by Neurospora crassa was worse than that of inulin,the proliferation effect of Bifidobacterium adolescentis CICC 6178 of oligosaccharides in soybean residue fermented by Neurospora crassa was better than that of inulin.