诺丽果、种子和叶提取物抑菌活性及复合植物抑菌液配方筛选

Study on antimicrobial properties of Noni fruit,seed and leaf extract and screening of antimicrobial composite formula

研究诺丽果、种子和叶提取物的抑菌活性。通过考察诺丽果发酵汁、种子乙醇提取物、种子水蒸馏提取物、诺丽叶提取物、金银花提取物分别对铜绿假单胞菌、大肠埃希菌、金黄色葡萄球菌、变形链球菌4种致病菌的抑菌活性,筛选出了抑菌活性较强的3种提取物(诺丽果发酵汁、种子水蒸馏提取物、金银花提取物)。通过单因素实验、3因素3水平正交实验,优化了复合植物抑菌液的最佳配方。结果表明:以诺丽发酵汁87%、种子水蒸馏提取物8%、金银花提取物5%制成的抑菌液,抑菌圈直径分别为:铜绿假单胞菌15 mm,大肠埃希菌22 mm,金黄色葡萄球菌28 mm,变形链球菌17 mm。抑菌效果较好。结论:复合植物抑菌液具有抑菌的作用,为进一步开发抑菌产品提供理论依据。

The paper researched the antimicrobial properties of extracts of Noni fruit,seed and leaf extract. By studying the antimicrobial properties of Noni fruit fermented juice,seed ethanol extract,seed steam distillate extract,Noni leaf extract and honeysuckle extract respectively on four kinds of pathogens as the target,Pseudomonas aeruginosa,Escherichia coli,Staphylococcus aureus,Streptococcus mutans,three kinds of extracts with stronger antimicrobial properties（ Noni fruit fermented juice,seed steam distillate extract and honeysuckle extract） were screened out.Through the experimental results from the single factor experiment and three factors three levels orthogonal experiments,the best formula of compound plant bacteriostatic liquid was optimized.The results showed that the antimicrobial effect of liquid mixed from 87% of the Noni fruit fermented juice,8%of the distilled steam extract and 5% of the honeysuckle extract was the best.The inhibition zone diameters of different bacteria were 15 mm of Pseudomonas aeruginosa,22 mm of Escherichia coli,28 mm of Staphylococcus aureus,17 mm of Streptococcus mutans.The compound plant bacteriostatic liquid has antimicrobial effect,and it provides theoretical basis for further developing antimicrobial product.