姜黄素对秀丽隐杆线虫降脂及抗氧化保护作用

Lipid-lowering and anti-oxidative effects of curcumin on Caenorhabditis elegans

本文研究姜黄素对秀丽隐杆线虫的降脂及抗氧化作用。将L1期秀丽隐杆线虫暴露于不同浓度姜黄素(12.5、25、50和100μmol/L)48 h后,以油红O染色线虫肠道脂肪,检测线虫体内甘油三酯的含量变化,同时测定线虫体内总超氧化物歧化酶活力(T-SOD)和丙二醛(MDA)含量的变化。结果表明,与二甲基亚砜(DMSO)对照组相比,姜黄素浓度在25μmol/L以上显著降低线虫肠道脂肪(p<0.05),姜黄素浓度在50μmol/L以上降低线虫肠道脂肪作用极显著(p<0.01),随着姜黄素浓度升高,线虫肠道脂肪含量逐渐降低。姜黄素浓度在25μmol/L以上极显著降低线虫体内甘油三酯和MDA含量(p<0.01)。黄素浓度在50μmol/L以上极显著提高线虫体内T-SOD酶活力(p<0.01)。综上所述,姜黄素浓度在25μmol/L以上对线虫具有降脂及抗氧化保护作用。

To study the lipid-lowering and anti-oxidative effects of curcumin on Caenorhabditis elegans, C.elegans in L, period were exposed to the different concentrations of curcumin （12.5,25,50 and 100 μmol/L）for 48 h. Oil red O stained nematode intestinal fat, the activities of T-SOD, the contents of MDA and triglyceride in C.elegans were measured. Resuhs showed that compared with DMSO control group, eurcumin could reduce intestinal fat in C.elegans at 25 μmol/L or more（p 〈 0.05 ）, curcumin could reduce intestinal fat significantly at 50 μmol/L or more （p 〈 0.01 ）, with the increasing of curcumin concentration,the content of intestinal fat decreased gradually.Curcumin could reduce the contents of triglyceride and MDA in C.elegans significantly at 25μmol/L or more （p 〈 0.01 ）. Cureumin could increase the activities of T- SOD in C.elegans significantly at 50 μmol/L or more（p 〈 0.01 ）. In summary, curcumin had lipid-lowering and antioxidant protection effects at 25 μmol/L or more.