摘要
为了探究沃尼妙林对脂多糖(LPS)诱导的急性肺损伤(ALI)Balb/c小鼠肺组织中TLR4和CD14表达的影响,试验运用Real time-PCR和Western-blot方法检测LPS诱导的Balb/c小鼠ALI肺组织中TLR4和CD14的表达水平。结果表明:与空白组相比,沃尼妙林处理ALI的Balb/c小鼠肺组织中TLR4和CD14的mRNA和蛋白表达水平均显著性降低(P<0.05);随着沃尼妙林剂量的升高,其差异显著性增强。说明沃尼妙林能够抑制LPS诱导的Balb/c小鼠ALI肺组织中TLR4和CD14的分子表达。
To investigate the effect of valnemulin in the process which LPS induces the expression of TLR4 and CD14 in the routine ALL With the methods of Real time PCR and Western - blot to detecte the expression of TLR4 and CD14 in ALI Balb/c mice. The experiment showed that valnemulin could obviously reduce mRNA and protein levels of TLR4 and CD14 in the murine ALI（ P 〈 0.05 ） ; compared with LPS -induced group, with the increace of valnemulin dosage, the differences significantly enhanced. The results indicated that valnemulin had anti - inflamma- tory properties on LPS -induced acute injury,and could significantly suppress the expression of TLR4 and CD14 in the LPS- induced Balb/c murine ALL
出处
《黑龙江畜牧兽医》
CAS
北大核心
2017年第7期27-30,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
黑龙江省农垦总局科技计划课题"寒区规模化猪场抗生素规范用药技术与示范"(HNK1350406)
黑龙江八一农垦大学博士科研启动计划项目(XDB2012-13)
