泄浊排毒方对慢性肾脏病大鼠模型蛋白结合型尿毒症毒素清除的影响

Effects of Xiezhuo Paidu Decoction on Protein Bound Uremia Toxin in Rats with Chronic Kidney Disease

目的观察泄浊排毒方对慢性肾脏病模型大鼠代谢毒素尤其是蛋白结合型毒素的影响及优势。方法将32只SD大鼠随机分为4组:空白组、模型组、腹膜透析组、泄浊排毒方组,各组按相应药物剂量灌胃,腹透;造模第8周末检测各组血清尿素、肌酐、尿酸、胱抑素C含量,运用高效液相色谱-荧光检测法检测血清硫酸吲哚酚含量。结果与空白对照组相比,模型组血清尿素、肌酐含量明显升高(P<0.05);与模型组相比,泄浊排毒方组尿素、肌酐、尿酸、胱抑素C含量明显降低(P<0.05);与腹透组相比,泄浊排毒方组尿素、胱抑素C、尿酸含量明显降低(P<0.05);与模型组相比,泄浊排毒方组硫酸吲哚酚含量明显降低(P<0.05);与腹透组相比,泄浊排毒方硫酸吲哚酚含量明显降低(P<0.05)。结论泄浊排毒方可清除尿毒症毒素,延缓肾损害进展,其原因可能与蛋白结合型代谢毒素的清除有关。

Objective： To study the regulatory effects and advantages of Xiezhuo Paidu Decoction on protein bound uremia toxin in rats with chronic kidney disease. Methods： 32 SD rats were divided in to the blank group,the model group,peritoneal dialysis group and Xiezhuo Paidu Decoction group. Each group received gavage and peritoneal dialysis according to the corresponding dose. After 56 days＇ treatment,serum urea,creatinine,uric acid,Cystatin C were measured,and the content of indoxyl sulfate in serum was determined by HPLC-MS. Results：Compared with the blank control group,the serum urea and creatinine levels in the model group increased significantly（P 0.05）. Compared with the model group,the contents of urea,creatinine,uric acid and cystatin C decreased significantly in Xiezhuo Paidu Decoction group（P 0.05）. Compared with the peritoneal dialysis group,the contents of urea,cystatin C and uric acid decreased significantly in Xiezhuo Paidu Decoction group（P 0.05）. Compared with the model group and the peritoneal dialysis group,the content of indoxyl-sulfate decreased obviously in Xiezhuo Paidu Decoction group（P 0.05）. Conclusions： Xiezhuo Paidu Decoction can clear uremic toxins and delay the progression of renal damage,which may be related to the elimination of protein bound metabolic toxins.