清肺方对类风湿性关节炎相关间质性肺炎模型大鼠的TGF-β_1作用的实验研究

Experimental Study on the Effect of Qingfei Decoction on TGF-β_1 in Rats with Rheumatoid Arthritis Associated Interstitial Pneumonia

目的通过博来霉素诱导建立类风湿性关节炎相关间质性肺炎大鼠模型,探讨清肺方对间质性肺炎大鼠的作用及其对TGF-β_1的作用。方法清洁级SD大鼠48只,雄性,体质量为160~180 g,适应性饲养1周后,通过气管内灌注3 mg/mL博来霉素诱导建立大鼠间质性肺炎动物模型,分为清肺方组,间质性肺炎模型组,正常对照组,强的松组4组,各12只。用药28 d后,对比清肺方组与间质性肺炎模型组、强的松组、正常对照组之间的肺组织染色及纤维化评分,肺组织染色测量胶原面积,碱水解法检测肺组织含量,检测血清及肺组织TGF-β_1。结果1)清肺方组大鼠肺组织纤维化程度、羟脯氨酸浓度均显著低于间质性肺炎模型组(P<0.01)。2)清肺方组大鼠血清TGF-β_1浓度低于间质型肺炎模型组(P<0.01)。3)清肺方组大鼠肺组织匀浆TGF-β_1浓度低于间质性肺炎模型组(P<0.01)。结论 1)清肺方对博来霉素诱导的大鼠间质性肺炎、肺纤维化有抑制作用。2)清肺方对博来霉素诱导的大鼠间质性肺炎的抑制作用机制可能与TGF-β_1有关。

Objective： To establish a rat model of rheumatoid arthritis associated interstitial pneumonia induced by bleomycin,and to explore the effect of Qingfei Decoction on interstitial pneumonia and its effect on TGF-β1.Methods： 48 male SD rats,weight 160~180 g,were fed adaptively for one week and the rat model of interstitial lung disease were established by means of pour 3 mg/kg BLM into trachea and divided into 4 groups ： Qingfei Decoction group,the interstitial pneumonia model group,the normal control group and predmisone group,12 in each.After 28 days′ medication,lung tissue staining and fibrosis scores were compared among all groups. The area of collagen was measured by lung staining,and the content of lung tissue was detected by alkaline hydrolysis. TGF-β1 in serum and lung tissues were detected. Results： 1）The degree of fibrosis and hydroxyproline concentration in lung tissue in Qingfei Decoction group were obviously lower than those of the interstitial pneumonia model group（P 0.01）. 2）Concentration of serum TGF-beta 1 in Qingfei Decoction group was lower than that of the interstitial pneumonia model group（P 0.01）. 3）Concentration of TGF-beta 1 of Lung homogenate in Qingfei Decoction group was lower than the interstitial pneumonia model group（P 0.01）. Conclusion： 1）Qingfei Decoction has an inhibitory effect on bleomycin induced interstitial pneumonia and pulmonary fibrosis in rats. 2）The treatment mechanism of Qingfei Decoction to BLM-ILD may be related to TGF-beta1.