推拿对压力性尿失禁大鼠耻尾肌肌纤维的影响

Effect of Massage on Pubococcygeus Muscle Fibers of Stress Urinary Incontinence Rats

目的观察推拿对压力性尿失禁大鼠耻尾肌肌纤维的影响,探讨推拿治疗女性压力性尿失禁的机理。方法将产后SD大鼠40只随机分为推拿治疗组、模型对照组,另设健康未生育大鼠20只为空白对照组。选用阴道球囊扩张术加双侧卵巢切除术进行造模,对推拿组施以腰骶部和腹部手法治疗,每日1次,共30次。然后处死所有大鼠,取耻尾肌组织,经HE染色,在光镜下采集并分析图像,比较各组耻尾肌肌纤维的差异。结果推拿治疗组耻尾肌肌纤维宽度、面积、直径更接近空白对照组,与模型对照组比较,差异有统计学意义(P<0.001),推拿治疗组及空白对照组比较无显著性差异(P>0.05)。结论推拿治疗组可使耻尾肌肌肉纤维增粗,从而使控尿能力有所提高,这为推拿治疗SUI提供了理论依据。

Objective： To observe the effect of massage on pubococcygeus muscle fiber of stress urinary incontinence rats,and to explore the mechanism of massage on female stress urinary incontinence. Methods： 40 postpartum SD rats were randomly divided into the massage treatment group and the model group,and 20 healthy rats without birth were used as the blank group. The models were made with the vaginal balloon dilatation plus bilateral oophorectomy. The massage treatment group was treated with manipulation of the lumbosacral region and abdomen,once a day,30 times. Then all the rats were killed;the pubococcygal muscle tissue was taken. Pubococcygal muscle sections were stained with HE,and under the light microscope image collected and analyzed,and the differences of the pubococcygeus muscle fibers in all groups were compared. Results： The width,area and diameter of pubococcygeus muscle fiber of the massage treatment group were close to those of the blank group and compared with model group,the difference was statistically significant（P 0.001）. There was no significant difference between the massage treatment group and the blank group（P 0.05）. Conclusion： Massage can make the pubococcygeus muscle fiber thickening,so as to improve the urine control ability,and provide a theoretical basis for the massage treatment on SUI.