下调CK2α基因表达抑制肺腺癌A549细胞的增殖和凋亡

Down-regulation of CK2α expression inhibits the proliferation and apoptosis of A549 cell line in lung adenocarcinoma

目的:探讨下调CK2α基因表达后对肺腺癌A549细胞的影响。方法:构建p SilencerTM4.1-sh CK2α-e GFP慢病毒表达载体,建立稳定干扰CK2α表达的A549细胞株。利用MTT、克隆形成、凋亡实验检测干扰CK2α基因表达后,A549细胞的增殖和凋亡的能力。利用Western blot检测细胞凋亡相关蛋白Caspase-3、Caspase-8、Bcl-xl、Bcl-2的表达。结果:下调CK2α基因表达后肺腺癌A549细胞增殖能力减低,促进细胞凋亡。细胞凋亡相关蛋白Caspase-3、Caspase-8上调,但Bcl-xl、Bcl-2蛋白明显下调。结论:下调CK2α基因表达后抑制肺腺癌A549细胞增殖、促进凋亡。

Objective：To investigate the effect of A549 cell line after down-regulation of protein kinase CK2α expression in lung adenocarcinoma.Methods：We constructed pSilencerTM4.1-shCK2α-eGFP of lentiviral-mediated shRNA.Then we knock-downed expression of CK2α gene and established stable A549 cell line by lentivirus-mediated shRNA.Analyzing the proliferation and apoptosis capacities of A549 cell line by MTT assay,colony formation assay and apoptosis assay.Moreover,we examined proteins expression of Caspase-3,Caspase-8,Bcl-xl and Bcl-2 by Western blot.Results：A549 cell line of down-regualted CK2α expression showed lower proliferation and more promotable apoptosis than control groups.Furthermore,compared to control groups,proteins of Caspase-3,Caspase-8 were significantly up-regulated and Bcl-xl,Bcl-2 were significantly down-regulated.Conclusion：Silence of CK2α expression would inhibit proliferation and activate apoptosis in lung adenocarcinoma A549 cell line.