基因测序技术在细菌性传染病监测中的应用

Application of gene-sequencing technique in the monitoring of bacterial infectious diseases

基因测序技术是现代分子生物学研究中最为重要的技术手段,经过数十年发展,以其通量高、速度快及准确性高而被广泛应用于疾病检测和监测中。为此,对基因测序技术原理和发展历程进行综述,系统介绍目前常见的一代、二代及三代测序技术方法的原理以及基于不同技术原理的各类测序设备,探讨各类测序设备在细菌性传染病应急和预警监测过程中的选择和应用,并对测序技术在生物学和生物医学研究中应用发展进行综述和展望。

Gene-sequencing technique is the most important techniques in the research of modern molecular biology. After decades of development, it has been widely used in disease detection and monitoring due to its high throughput, fast speed and high accuracy. Therefore, the principle and development process of gene-sequencing technique were summarized in this paper, and the principle of gene-sequencing technique of the first generation, the second generation and the third generation and all kind of gene-sequencing equipment based on various principle of technique were systematic introduced in this paper. Besides, the paper also explored and discussed the selection and application of various kind of gene-sequencing equipment in the emergency and early warning monitoring for bacterial infectious disease. On the other hand, the paper also summarized and prospected the application and development of gene-sequencing technique in biology and biomedical research.