CIDEC的亚细胞定位及其功能初步研究

Localization and Preliminarily Functional Analysis of CIDEC

CIDEC(也称FSP27)高表达于脂肪组织,可以促进细胞内脂肪积累等。为探究CIDEC促进脂滴融合的机制,本研究利用脂肪酸处理HepG2细胞,测定细胞内脂滴直径和数目的变化,发现处理后脂滴的直径和数量无显著性差异。将含有CIDEC的重组载体转染细胞,脂肪酸处理24 h后测定脂滴直径和数量变化,进一步利用共聚焦显微镜分析其亚细胞定位,并检测与脂滴生成、生长等相关基因表达量的变化。结果表明,过表达CIDEC后脂滴的直径显著增加,脂滴的数量极显著减少;亚细胞定位发现,CIDEC位于脂滴周围。此外,PLIN1、CREB1、CREB8的表达量有所下降,CIDEA、CFD表达量有所上升,推测CIDEC可能与这些蛋白互作引起脂滴融合。本研究结果为探究CIDEC促进脂滴融合的分子机制奠定了基础。

CIDEC（ also known as FSP27） is highly expressed in adipose tissue that promotes intracellular triglyceride（ TAG） storage. To explore the mechanism of CIDEC promoting lipid droplet fusion,it had been studied that the diameter and the number of lipid droplets in Hep G2 cells incubated with fatty acid were detected,and the result found there were no significant difference before or after treatments. After cells transfected with recombinant vector containing CIDEC was incubated in fatty acid for 24 h,the diameter and number of lipid droplets were measured,its subcellular localization were analyzed by confocal microscopy,and expression of genes related to lipid droplet formation and growth were detected. It indicated that the diameter of lipid droplets increased and the number decreased significantly after overexpression of CIDEC. Moreover,the expressions of PLIN1,CREB1,CREB8 were decreased,while the expressions of CIDEA,CFD were increased. It is speculated that CIDEC may interact with these proteins to cause lipid droplet fusion.This study provides theoretical basis study the mechanism of lipid droplet fusion promoting by CIDEC.