L-型钙通道阻滞剂对吗啡奖赏记忆再巩固的影响

EFFECTS OF L-TYPE CALCIUM CHANNEL ANTAGONIST ON RECONSOLIDATION OF MORPHINE REWARD MEMORY

目的:确定L-型钙通道阻滞剂对吗啡奖赏记忆再巩固的影响。方法:采用大鼠条件性位置偏爱模型,条件性线索暴露后,单次系统给予硝苯地平和维拉帕米,观察其对吗啡奖赏记忆条件位置偏爱分值的影响,同时检测对自发活动的影响。结果:维拉帕米组与对照组相比,条件性位置偏爱分值明显降低,统计学差异具有显著性(P<0.05),其自发活动统计学差异无显著性(P>0.05)。硝苯地平组与其对照组相比,条件性位置偏爱分值统计学差异无显著性(P>0.05),硝苯地平组自发活动降低(P<0.05),差异有显著性。结论:维拉帕米可干扰药物奖赏记忆再巩固的过程,且对大鼠自发活动没有影响,具有潜在的戒毒防复吸的临床应用前景,而硝苯地平对奖赏记忆作用不明显,且加大剂量明显抑制大鼠自发活动,其戒毒防复吸作用受到限制。

Objective： To explore the effects of L-type calcium channel antagonist on the reconsolidation of morphine reward memory in rats.Methods： Sprague Dawley（ SD） rats were used in the study. Using a conditioned place preference（ CPP） procedure,the effects of nifedipine and verapamil systemically administered on reconsolidation of morphine reward memory were investigated after re-exposure to drug-paired environment. The effects of nifedipine and verapamil on the locomotor were investigated immediately after systemically administration.Results： The CPP score was significantly lower in the verapamil group than in the control group（ P 0. 05）,while there was no significant difference between the nifedipine group and the control group（ P 0. 05）. Compared with the control group,the locomotor of nifedipine group was decreased（ P 0. 05）,and there was no significant difference in the verapamil group（ P 0. 05）.Conclusion： Verapamil can play a critical role in the treatment of drug addiction via interfering with the reconsolidation of drug reward memory,meantime,it does not affect locomotor. However,nifedipine did not achieve the desired effect,and will inhibit locomotor when the dose was increased.