银杏总黄酮对非酒精性脂肪性肝病大鼠肝细胞凋亡的影响及其机制的研究

Research on the effect and mechanism of total ginkgo flavones-glycoides on hepatocyte apoptosis in rats with nonalcoholic fatty liver disease

[目的]研究银杏总黄酮对非酒精性脂肪性肝病(NAFLD)大鼠肝细胞凋亡的影响,并检测大鼠肝脏Caspases 3蛋白磷酸化水平的表达,探讨银杏总黄酮抑制肝细胞损伤的作用机制。[方法]利用高脂饲料喂养法构建NAFLD大鼠模型。SPF级雄性大鼠分为正常对照组、NAFLD模型组和银杏总黄酮治疗组3组。光镜检查大鼠肝组织病理形态变化,细胞原位TUNEL法检测肝细胞凋亡率。Western-blot法检测Caspase 3蛋白磷酸化水平。[结果]成功构建NAFLD大鼠模型。TUNEL法检测结果显示第4周后,NAFLD模型组和银杏总黄酮治疗组大鼠肝细胞凋亡较正常对照组增加,Western-blot法检测结果显示Caspase 3蛋白磷酸化水平较正常对照组增加。在第8周、第12周后,模型组肝细胞凋亡数目逐渐增多,Caspase 3蛋白磷酸化水平逐渐增强;银杏总黄酮治疗组大鼠肝细胞凋亡逐步降低,Caspase 3蛋白磷酸化水平逐步下降。[结论]银杏总黄酮抑制NAFLD大鼠肝细胞凋亡,对NAFLD大鼠肝细胞有保护作用,其机制可能是通过降低Caspase 3蛋白磷酸化水平实现的。

[Objective]To research the effect of total ginkgo flavones-glycoides on hepatocyte apoptosis in rats with nonalcoholic fatty liver disease and assess the expression of Caspase 3 phosphorylation protein.[Methods]72 rats were chosen for the experiment which were randomly divided into the normal control group,the NAFLD model group and total ginkgo flavones-glycoides group.Rats were received the high fat diet for the induction of fatty liver.We applied HE method to detect the changing of NAFLD ratsliver histopathology and cell apoptosis was detected in liver tissue by TUNEL.We applied Western-blot method to detect the expression of Caspase 3 phosphorylation protein.[Results]The model of nonalcoholic fatty liver disease was developed.In the fourth weekend,the cell apoptosis and the expression of Caspase 3 phosphorylation in the NAFLD model group and total ginkgo flavones-glycoides group were higher than those of the normal control group.With the progress of NAFLD,the level of the cell apoptosis and the expression of Caspase 3 phosphorylation increased gradually in the NAFLD model group,while no significant changewas found in the total ginkgo flavones-glycoides group.[Conclusion]The total ginkgo flavones-glycoides decoction can prevent the hepatocyte apoptosis in the NAFLD rats,which may be through decreasing the lever of Caspase 3 phosphorylation protein.